Several bovine and ovine antibodies against cytokines have been tested and shown to be suitable for caprine research.
Our antibodies against bovine IFN gamma and bovine IL-4 are well characterized, and are shown to cross-react with goat. Our anti-ovine IL-1 beta monoclonal and anti-ovine IL-8 polyclonal antibodies are also suitable for use with caprine samples.
Measurement of interferon gamma (IFN-γ) and interleukin-4 (IL-4) plays an important role in evaluating the immune status of animals. IFN-γ is a secreted, pro-inflammatory cytokine, produced by lymphocytes in response to activation by specific antigens or mitogens. IL-4 plays a critical role in influencing antigen-activated naïve CD4+ T cells to develop into Th2 cells. Th2 cells are associated with specific immune responses, including those against large extracellular parasites.
Our anti-bovine IL-4 antibodies, clones CC313 and CC314, are suitable for use in sandwich ELISA. A publication in Research in Veterinary Science cites the use of these antibodies to measure soluble IL-4 in caprine PBMC culture supernatants.
Marinaro M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.
Research in Veterinary Science 93: 662–667
For recommendations for an ELISA protocol for this IL-4 antibody pair, see the information associated with our Bovine IL-4 ELISA Kit, which uses the same clones.
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