ELISA Synblock

//www.bio-rad-antibodies.com/elisa-synblock-accessory-reagent-buf034a.html?RenderPageSpecific=1
ELISA Synblock gallery image 1

Published customer image:
Synblock solution (BUF034) used for blocking non-specific binding sites in a Mincle binding assay
Image caption:
T. forsythia S-layer binds Mincle.
(A) Binding of S-layer to Mincle. T. forsythia S-layer immobilized in microtiter plate wells was incubated with Mincle-Fc fusion protein or MMR-Fc in lectin binding buffer (TBS, 5 mM CaCl2). As control, ManLAM was immobilized in wells. After washing wells with buffer, binding was detected with HRP-coupled goat anti-human IgG-Fc and chromogenic substrate TMB. Color was read by measuring absorbance at 450 nm. As shown, S-layer bound Mincle in a dose dependent manner and MMR as control bound ManLAM but not S-layer. (B) Mincle binding to T. forsythia S-layer is Ca2+-dependent. To analyze the Ca2+ dependency of Mincle binding to S-layer, Mincle-Fc was incubated with plate bound S-layer in Ca2+ (TBS, 5 mM CaCl2) or EDTA containing buffer (TBS, 10 mM EDTA). Plate bound TDB was used a control ligand or Mincle binding. Binding was then detected as above. (C) S-layer dose dependently inhibited binding of Mincle to TDB. Plate bound TDB was incubated with Mincle-Fc in lectin binding buffer with increasing concentrations of S-layer. Wells were washed with elution buffer and binding was measured as above. Each value represents the mean (± SD) of 3 values measured in one representative assay. Data are representative of three independent experiments with similar results; *, P<0.05.

From: Chinthamani S, Settem RP, Honma K, Kay JG, Sharma A (2017)
Macrophage inducible C-type lectin (Mincle) recognizes glycosylated surface (S)-layer of the periodontal pathogen Tannerella forsythia.
PLoS ONE 12(3): e0173394.

Enlarge
  • ELISA Synblock thumbnail image 1
  • ELISA Synblock
  • ELISA Synblock
  • ELISA Synblock
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF034AEdatasheet pdfdatasheet pdf100 ml
    BUF034A
    BUF034CEdatasheet pdfdatasheet pdf1000 ml
    BUF034C
    BUF034BEdatasheet pdfdatasheet pdf500 ml
    BUF034B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • ELISA Synblockis an ELISA buffer designed to avoid false positive results associated with buffers containing animal proteins (e.g. BSA) and reduce non-specific background signals in ELISA assays without the addition of the usuall protein additives.
    • Intended Use
      Pack Size: 1000 mlELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With detergent and synthetic blocking agents, the inert nature of this unique buffer enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

      Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

      N.B. SynBlock is not suitable for use on Immunlon® 2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
      Pack Size: 100 ml, 500 mlELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

      Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

      N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
      Pack Size: 1000 mlELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With detergent and synthetic blocking agents, the inert nature of this unique buffer enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

      Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

      N.B. SynBlock is not suitable for use on Immunlon® 2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
      Pack Size: 100 ml, 500 mlELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

      Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

      N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
      Pack Size: 1000 mlELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With detergent and synthetic blocking agents, the inert nature of this unique buffer enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

      Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

      N.B. SynBlock is not suitable for use on Immunlon® 2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
      Pack Size: 100 ml, 500 mlELISA SynBlock is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.

      Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.

      N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. Bio-Rad recommends the use of BUF033A for this purpose.
    • Product Form
      Ready to use - liquid
      Ready to use - liquid
      Ready to use - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.05%Sodium Azide
      0.05%Sodium Azide
      0.05%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE
      Store at +4oC.

      DO NOT FREEZE
      Store at +4oC.

      DO NOT FREEZE
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1. Coat ELISA plate with antibody or antigen as required.

      2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

      3. Add 300-400ul of BUF034C and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

      4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
    • Instructions For Use
      1. Coat ELISA plate with antibody or antigen as required.

      2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

      3. Add 300-400ul of BUF034A and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

      4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
    • Instructions For Use
      1. Coat ELISA plate with antibody or antigen as required.

      2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. BUF031A can be used for this purpose.

      3. Add 300-400ul of BUF034B and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.

      4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.

    Additional ELISA Synblock Formats

    Formats Applications Sizes available
    ELISA Synblock : Ready To Use E 500 ml | 100 ml | 1000 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

              Write your review

               

              You may also be interested in...