ELISA Neptune Assay Diluent

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ELISA Neptune Assay Diluent gallery image 1

Published customer image:
Neptune ELISA diluent buffer (BUF039) used to dilute serum samples used in an ELISA assay designed to measure gonadotropin-releasing hormone levels in cattle following immunization.
Image caption:
Mean (SD) anti-GnRH antibody titres for cattle treated with the immunocontraceptive vaccine GonaCon. All cattle received a single dose of GonaCon, WB received a booster dose three months later.

From: Massei G, Koon K-K, Benton S, Brown R, Gomm M, Orahood DS, et al. (2015)
Immunocontraception for Managing Feral Cattle in Hong Kong.
PLoS ONE 10(4): e0121598.

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  • ELISA Neptune Assay Diluent
  • ELISA Neptune Assay Diluent
  • ELISA Neptune Assay Diluent
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF039AEdatasheet pdfdatasheet pdf100 ml
    BUF039A
    BUF039CEdatasheet pdfdatasheet pdf1000 ml
    BUF039C
    BUF039BEdatasheet pdfdatasheet pdf500 ml
    BUF039B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Product Form
      Ready to use - liquid
      Ready to use - liquid
      Ready to use - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1%Sodium Azide
      0.1%Sodium Azide
      0.1%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE
      Store at +4oC.

      DO NOT FREEZE
      Store at +4oC.

      DO NOT FREEZE
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISANeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Pipette buffer directly onto the plate just prior to adding the sample or standard (suggested volume 50 - 100ul/well).
    • Instructions For Use
      Pipette buffer directly onto the plate just prior to adding the sample or standard (suggested volume 50 - 100ul/well).
    • Instructions For Use
      Pipette buffer directly onto the plate just prior to adding the sample or standard (suggested volume 50 - 100ul/well).

    Additional ELISA Neptune Assay Diluent Formats

    Formats Applications Sizes available
    ELISA Neptune Assay Diluent : Ready To Use E 1000 ml | 100 ml | 500 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          ELISA UltrablockBUF033A100 mlE
          BUF033A
          ELISA UltrablockBUF033C1000 mlE
          BUF033C
          ELISA UltrablockBUF033B500 mlE
          BUF033B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          ELISA UltrablockBUF033A100 mlE
          BUF033A
          ELISA UltrablockBUF033C1000 mlE
          BUF033C
          ELISA UltrablockBUF033B500 mlE
          BUF033B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          5x ELISA Coating BufferBUF030A100 mlE
          BUF030A
          10x ELISA Wash BufferBUF031A100 mlE
          BUF031A
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          ELISA UltrablockBUF033A100 mlE
          BUF033A
          ELISA UltrablockBUF033C1000 mlE
          BUF033C
          ELISA UltrablockBUF033B500 mlE
          BUF033B

          Recommended Positive Controls

            Histology Controls

              References

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