CYTOTRACK™

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Resolution of ten cell generations using CytoTrack 511/525

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  • CYTOTRACK™ thumbnail image 1
  • CYTOTRACK™ Blue 403/454 Cell Proliferation Assay Kit
  • CYTOTRACK™ Yellow 542/556 Cell Proliferation Assay Kit
  • CYTOTRACK™ Green 511/525 Cell Proliferation Assay Kit
  • CYTOTRACK™ Red 628/643 Cell Proliferation Assay Kit
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  • Product Type
    Accessory Reagent
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1351202Fdatasheet pdfdatasheet pdf200 Tests
    1351202
    1351204Fdatasheet pdfdatasheet pdf200 Tests
    1351204
    1351203Fdatasheet pdfdatasheet pdf200 Tests
    1351203
    1351205Fdatasheet pdfdatasheet pdf200 Tests
    1351205
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • CytoTrack cell proliferation assay kits are available in four distinct dyes for easy multicolor cell analysis: blue, green, yellow and red. Easily incorporate a cell tracking stain into your multicolor panel.

      The proprietary chemistry of CytoTrack dyes enables the resolution of up to ten cell divisions. Each dye is cell permeable and comprises a fluorophore, a fluorescence blocker and a cell-retaining group. Upon entering a live cell, the fluorescence blocker is cleaved by intracellular esterases and the cell-retaining group of the fluorophore reacts with intracellular proteins to create a stable, covalent bond. As the cells divide, the fluorescence intensity is successively halved and each cell divison can be identified.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      CytoTrack Dye (4 vials, 50 assays/vial)
      DMSO (1 vial, 250 μl)
      CytoTrack Dye (4 vials, 50 assays/vial)
      DMSO (1 vial, 250 μl)
      CytoTrack Dye (4 vials, 50 assays/vial)
      DMSO (1 vial, 250 μl)
      CytoTrack Dye (4 vials, 50 assays/vial)
      DMSO (1 vial, 250 μl)
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only

      This product is photosensitive and should be protected from light
      Store at -20oC only

      This product is photosensitive and should be protected from light
      Store at -20oC only

      This product is photosensitive and should be protected from light
      Store at -20oC only

      This product is photosensitive and should be protected from light
    • Shelf Life
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Important: Thaw all components prior to use.

      1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

      2.Protocol for use in culture medium - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

      Protocol for use with buffer - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

      3. Incubate at room temperature for 15 mins. Protect from light.

      4. Pellet the cells by centrifugation.

      5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

      6. Resuspend the cell in 500 μl of culture media.

      7. Place the cells in the appropriate conditions for cells proliferation.

      8. Harvest the cells and stain them for other markers if appropriate.

      9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
    • Instructions For Use
      Important: Thaw all components prior to use.

      1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

      2.Protocol for use in culture medium - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

      Protocol for use with buffer - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

      3. Incubate at room temperature for 15 mins. Protect from light.

      4. Pellet the cells by centrifugation.

      5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

      6. Resuspend the cell in 500 μl of culture media.

      7. Place the cells in the appropriate conditions for cells proliferation.

      8. Harvest the cells and stain them for other markers if appropriate.

      9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
    • Instructions For Use
      Important: Thaw all components prior to use.

      1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

      2.Protocol for use in culture medium - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

      Protocol for use with buffer - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

      3. Incubate at room temperature for 15 mins. Protect from light.

      4. Pellet the cells by centrifugation.

      5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

      6. Resuspend the cell in 500 μl of culture media.

      7. Place the cells in the appropriate conditions for cells proliferation.

      8. Harvest the cells and stain them for other markers if appropriate.

      9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.
    • Instructions For Use
      Important: Thaw all components prior to use.

      1. Prepare a 500x stock solution. Add 50 μl of DMSO and mix.

      2.Protocol for use in culture medium - Add 1 μl of stock solution into 500 μl of media containing 1 x 106 cells of interest.

      Protocol for use with buffer - Prepare a 1x working solution. Add 1 μl of stock solution into 500 μl of buffer, pH 7. Add 500 μl of 1x solution to 1 x 106cells.

      3. Incubate at room temperature for 15 mins. Protect from light.

      4. Pellet the cells by centrifugation.

      5. Remove the supernatant and wash the cells using 3 ml of fresh prewarmed culture media.

      6. Resuspend the cell in 500 μl of culture media.

      7. Place the cells in the appropriate conditions for cells proliferation.

      8. Harvest the cells and stain them for other markers if appropriate.

      9. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with the appropriate excitation and emission filters.

    Additional CYTOTRACK™ Formats

    Formats Applications Sizes available
    CYTOTRACK™ : 628/643 F 200 Tests
    CYTOTRACK™ : 511/525 F 200 Tests
    CYTOTRACK™ : 542/556 F 200 Tests
    CYTOTRACK™ : 403/454 F 200 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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