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Rat IgG2a Negative Control antibody

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Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 1 Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 2 Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 3 Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 4 Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 thumbnail image 5
Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 1
Figure A. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat anti mouse CD40 (MCA1143PE). All experiments performed on mouse splenocytes in the presence of mouse SeroBlock (BUF041A)
Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 2
Figure A. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat IgG2a isotype control (MCA1212A488). Figure B. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat anti Mouse CD45R. All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)
Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 3
Figure A. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Rat anti Mouse CD45R (MCA1258F) and RPE conjugated Rat anti Mouse CD48 (MCA925PE). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)
Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 4
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat anti Canine CD4 (MCA1038PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Rat IgG2a Neg Cont Antibody :Alexa Fluor 405 gallery image 5
Figure A. RPE conjugated Rat anti Canine CD5 (MCA1037PE) and A647 conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated Rat anti Canine CD5 (MCA1037PE) and A647 conjugated Mouse anti Canine CD4 (MCA1038A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Rat IgG2a Negative Control:Pacific Blue®

Product Type
Negative/Isotype Control
Isotype
IgG2a
Specificity
Rat IgG2a Negative Control

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MCA1212PB
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Rat IgG2a Negative Control antibody is suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells.

Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with canine cells.

N.B. This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.		 Isotype controls savings up to 65%

Target Species
Negative Control
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Human lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
The Pacific Blue dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl.
It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Rat IgG2a Negative Control antibody

  1. Stapleton, T.W. et al. (2000) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications.
    Tissue Eng Part A. 17: 231-42.
  2. Guilloteau, L.A. et al. (2003) Nramp1 is not a major determinant in the control of Brucella melitensis infection in mice.
    Infect Immun. 71: 621-8.
  3. Sumagin, R. et al. (2008) Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.
    Am J Physiol Heart Circ Physiol. 295: H969-H977.
  4. McConnell, M.J. et al. (2009) H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning.
    Proc Natl Acad Sci U S A. 106: 6784-9.
  5. Chiu, W.C. et al. (2011) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
    J Nutr Biochem. 20: 254-60.
  6. Schmidt, E.P. et al. (2012) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
    Nat Med. 18 (8): 1217-23.
  7. Park, S.W. et al. (2012) A1 adenosine receptor allosteric enhancer PD-81723 protects against renal ischemia-reperfusion injury.
    Am J Physiol Renal Physiol. 303: F721-32.
  8. Rabadi, M. et al. (2016) Peptidyl arginine deiminase-4-deficient mice are protected against kidney and liver injury after renal ischemia and reperfusion.
    Am J Physiol Renal Physiol. 311 (2): F437-49.
    9. View The Latest Product References
  9. Rabadi, M.M. et al. (2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO.
    Am J Physiol Renal Physiol. 316 (6): F1180-F1190.
  10. Han, S.J. et al. (2020) Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury.
    JCI Insight. 5 (19): e139246.
  11. Ono, Y. et al. (2018) CD11c+ M1-like macrophages (Møs) but not CD206+ M2-like Mø are involved in folliculogenesis in mice ovary.
    Sci Rep. 8 (1): 8171.
  12. Han, S.J. et al. (2020) Selective nanoparticle-mediated targeting of renal tubular Toll-like receptor 9 attenuates ischemic acute kidney injury.
    Kidney Int. 98 (1): 76-87.

Flow Cytometry

RRID
AB_567384

MCA1212PB

153556 164913 1710

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