Rat IgG2a/IgG1 Negative Control antibody
No associated image currently available
Rat IgG2a:FITC/Rat IgG1:RPE Negative Control
- Product Type
- Negative/Isotype Control
- Isotype
- IgG1
- IgG2a
- Specificity
- Rat IgG2a/IgG1 Negative Control
Antibody Quality and Performance Guarantee
| DC057 is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2a and IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. Suitable for use as negative control for human, pig, dog and mouse. |
- Target Species
- Negative Control
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Preparation
- Purified IgG from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgG2a (RAT)
RPE reagent: IgG1 (RAT) - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul to label 106 cells or 100ul whole blood
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
DC057
1703
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Negative Control ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up