Rat IgG1 Negative Control antibody

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Rat IgG1 Negative Control Antibody - HRP thumbnail image 2

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Rat IgG1 Negative Control Antibody - HRP gallery image 1 — Figure A. A488 conjugated Hamster anti Mouse CD3 (MCA2690A488) and RPE conjugated Rat IgG1 isotype control (MCA6004PE). Figure B. A488 conjugated Hamster anti Mouse CD3 (MCA463A488) and RPE conjugated Rat anti Mouse CD22 (MCA2187PE). Alll experiments performed on mouse splenocytes in the presence of murine Seroblock (BUF041A).

Rat IgG1 Negative Control Antibody - HRP gallery image 2 — Figure A. PE-A750 tandem-conjugated Rat anti Mouse CD45R (MCA1258P750) and RPE conjugated Rat IgG1 isotype control (MCA6004PE). Figure B. PE-A750 tandem-conjugated Rat anti Mouse CD45R (MCA1258P750) and RPE conjugated Rat anti Mouse CD150 (MCA2274PE). All experiments performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A).

Rat IgG1 Negative Control Antibody - HRP gallery image 3 — Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG1 isotype control (MCA6004PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat anti Canine CD8 (MCA1039PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Rat IgG1 Negative Control Antibody - HRP gallery image 4 — Figure A. Purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG1 FITC isotype control (**MCA6004F**). Figure B. Purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat anti Canine CD8 FITC (MCA1039F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG1 Negative Control:Amethyst Orange

Product Type: Negative/Isotype Control
Isotype: IgG1
Specificity: Rat IgG1 Negative Control

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References (2)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Rat IgG1 negative control, a rat monoclonal raised against horseradish peroxidase, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG1 test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.

Target Species: Negative Control
Product Form: Purified IgG conjugated to Amethyst Orange - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Immunogen: Horseradish peroxidase
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Amethyst Orange 405 540
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
Amethyst Orange	405	540

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

References for Rat IgG1 Negative Control antibody

do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
Research in Veterinary Science. 145: 193-204.
Matralis, D.T. et al. (2023) Intracellular IFN-γ and IL-4 levels of CD4 + and CD8 + T cells in the peripheral blood of naturally infected (Leishmania infantum) symptomatic dogs before and following a 4-week treatment with miltefosine and allopurinol: a double-blinded, controlled and cross-sectional study.
Acta Vet Scand. 65 (1): 2.