Mouse IgG1/IgG1 Negative Control antibody
No associated image currently available
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control
- Product Type
- Negative/Isotype Control
- Isotype
- Cocktail
- Specificity
- Mouse IgG1/IgG1 Negative Control
Antibody Quality and Performance Guarantee
| Mouse IgG1 FITC / Mouse IgG1 RPE negative control is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. |
- Target Species
- Negative Control
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE) - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Mouse IgG1/IgG1 Negative Control antibody
-
Steele, J. et al. (2002) Detection of CD4(+)- and CD8(+)- T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma Interferon release.
J. Virol. 76: 6027 - 6036. -
Youn, S.W. et al. (2004) Cellular senescence induced loss of stem cell proportion in the skin in vitro.
J Dermatol Sci. 35: 113-23.
- RRID
- AB_322251
DC012
153582
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Negative Control ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up