Mouse IgG1/IgG1 Negative Control antibody

100% Secure

Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control

Product Type
Negative/Isotype Control
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC012 F 50 Tests
Mouse IgG1 FITC / Mouse IgG1 RPE negative control is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.

Product Details

Target Species
Negative Control
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of Mouse IgG1/IgG1 Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Product Specific References

References for Mouse IgG1/IgG1 Negative Control antibody

  1. Steele, J. et al. (2002) Detection of CD4(+)- and CD8(+)- T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma Interferon release.
    J. Virol. 76: 6027 - 6036.
  2. Youn, S.W. et al. (2004) Cellular senescence induced loss of stem cell proportion in the skin in vitro.
    J Dermatol Sci. 35: 113-23.