Mouse IgG1/IgG1 Negative Control antibody
Mouse IgG1 FITC / Mouse IgG1 RPE negative control is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.
- Target Species
- Negative Control
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of reconstitution.
- For research purposes only
Applications of Mouse IgG1/IgG1 Negative Control antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
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Product Specific References
References for Mouse IgG1/IgG1 Negative Control antibody
Steele, J. et al. (2002) Detection of CD4(+)- and CD8(+)- T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma Interferon release.
J. Virol. 76: 6027 - 6036.
Youn, S.W. et al. (2004) Cellular senescence induced loss of stem cell proportion in the skin in vitro.
J Dermatol Sci. 35: 113-23.