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Recombinant chicken interferon alpha used to treat chicken DF-1 fibroblastoid cells prior to infection with avian influenza virus.
Chicken IFITM3 has an antiviral activity in DF-1 chicken cells. The expression level and log fold change of chIFITM3 were measured using quantitative RT-PCR after stimulation with IFN-a and IFN-? or after preincubation with a nontargeting siRNA or one specific to chIFITM3 (A). The effect of knocking down endogenous chIFITM3 expression in DF-1 cells infected with influenza A virus (A/WSN/1933 [WSN/33]) was measured by flow cytometry using an antibody against nucleoprotein (NP) (B). P = 0.01, Student's t test. DF-1 cells transfected with chIFITM3-HA were infected by WSN. Expression of HA and NP was detected by flow cytometry (C and D), and viral titers were measured by PFU (E). Error bars represent standard deviations across each condition performed in triplicate.
From: Smith SE, Gibson MS, Wash RS, Ferrara F, Wright E, Temperton N, Kellam P, Fife M. Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssa viruses in vitro.
J Virol. 2013 Dec;87(23):12957-66.