IFN Alpha

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IFN Alpha gallery image 1

Published customer image:
Recombinant Chicken interferon alpha used to treat chicken, duck and turkey lung cells prior to infection by avian flu virus.
Image caption:
Recombinant ChIFN-a reduces avian influenza virus replication. Inhibition of avian influenza virus (H1N1 and H5N9) replication in primary lung cell cultures derived from chicken (A), duck (B), and turkey (C) after rChIFN-a (1000 U/ml) pretreatment in vitro. Cells were infected with A/turkey/Virginia/2009 H1N1 or A/turkey/Wisconsin/68 H5N9 at MOI 0.1. Supernatants were harvested at the times indicated and viral titers were determined following injection into SPF embryos. The mean (and standard deviations) of three independent experiments are shown. Different lowercase letters denote significance in titer following rChIFN-a treatment groups (within columns) (P < 0.05) as determined by one-way ANOVA. Statistical differences (P < 0.05) following treatment between virus groups are shown by lowercase letter.

From: Jiang H, Yang H, Kapczynski DR. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species.
Virol J. 2011 Sep 22;8:447.

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IFN Alpha gallery image 2

Published customer image:
Recombinant Chicken interferon alpha used to treat chicken, duck and turkey lung cells prior to infection by avian flu virus.
Image caption:
Recombinant ChIFN-a inhibits pH1N1 and H5N9 virus nuclear protein expression. Primary chicken, turkey, and duck lung cells were pretreated with or without rChIFN-a (1000 U/ml) for 18 h. Monolayers were infected with either H1N1 or H5N9 avian influenza virus (MOI = 0.1) for 1 h, and replaced with fresh media. After 24 hours, cells were fixed and viral antigens were reacted with mouse-derived monoclonal antibody (P13C11) specific for type A influenza virus nucleoprotein followed by detection with Texas Red-labeled goat anti-mouse IgG antibody. Magnification 400×.

From: Jiang H, Yang H, Kapczynski DR. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species.
Virol J. 2011 Sep 22;8:447.

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IFN Alpha gallery image 3

Published customer image:
Recombinant Chicken interferon alpha used to treat chicken, duck and turkey lung cells prior to infection by avian flu virus.
Image caption:
Reduced cytopathic effect following rChIFN-a pretreatment following AIV infection. Primary chicken lung cell monolayers were pretreated with 1000 U/ml of rChIFN-a and infected with either H1N1 of H5N9 at 0.1 MOI. Negative control cells include no treatment/no virus (A), and IFN-a only (B). Protection from cytopathic effect was observed in cells infected with virus only, H1N1 (C) and H5N9 (E), compared with IFN-a treated cells that were then infected with H1N1 (D) or H5N9 (F). At 24 hpi the monolayers were digitally photographed using an inverted microscope at 200× magnification (Olympus America Inc., Melville, NY).

From: Jiang H, Yang H, Kapczynski DR. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species.
Virol J. 2011 Sep 22;8:447.

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IFN Alpha gallery image 4

Published customer image:
Recombinant chicken interferon alpha used to treat chicken DF-1 fibroblastoid cells prior to infection with avian influenza virus.
Image caption:
Chicken IFITM3 has an antiviral activity in DF-1 chicken cells. The expression level and log fold change of chIFITM3 were measured using quantitative RT-PCR after stimulation with IFN-a and IFN-? or after preincubation with a nontargeting siRNA or one specific to chIFITM3 (A). The effect of knocking down endogenous chIFITM3 expression in DF-1 cells infected with influenza A virus (A/WSN/1933 [WSN/33]) was measured by flow cytometry using an antibody against nucleoprotein (NP) (B). P = 0.01, Student's t test. DF-1 cells transfected with chIFITM3-HA were infected by WSN. Expression of HA and NP was detected by flow cytometry (C and D), and viral titers were measured by PFU (E). Error bars represent standard deviations across each condition performed in triplicate.

From: Smith SE, Gibson MS, Wash RS, Ferrara F, Wright E, Temperton N, Kellam P, Fife M. Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssa viruses in vitro.
J Virol. 2013 Dec;87(23):12957-66.

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  • Recombinant Chicken Interferon Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PAP004FNdatasheet pdfdatasheet pdf50 µg
    PAP004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Chicken interferon alpha (IFN alpha) shares structural and biological properties with mammalian IFN1. Recombinant chicken IFN alpha (rChIFNa) has been shown to act as a highly potent anti-viral agent in vitro (Schultz, U. et al. 1995). rChIFNa also resulted in a noticeable delay in the progression of tumours in CC progressor chickens infected with Rous Sarcoma Virus, when admimistered intravenously (Plachy, J. et al. 1999).

      Recombinant chicken interferon alpha is the immunogen used to produce the polyclonal Rabbit anti Chicken interferon alpha antibody.
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified recombinant protein expressed in E.coli - liquid.
    • Reconstitution
    • Preparation
      Recombinant protein prepared from E.coli
    • Preservative Stabilisers
      None present
    • Activity
      1x107units/mg
    • Purity
      >85% by Ni chelate chromatography
    • Approx. Protein Concentrations
      Total protein concentration 1.0mg/ml
    • Protein Molecular Weight
      19kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      0.05M Sodium Acetate
      1M Sodium Chloride
      2M Urea
      10mM Beta-Mercaptoethanol
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of despatch.
    • GO Terms
      cytokine receptor binding
      defense response
      cytokine activity
      response to virus
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IFN Alpha Formats

    Formats Applications Sizes available
    IFN Alpha : Rec. Protein FN 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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