CD25 Antibody | AbD13504

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CD25 Antibody | AbD13504 gallery image 1

Chicken PBMCs (stimulated for 3 days with concanavalin A) stained with Human anti Chicken CD25 (HCA173)

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CD25 Antibody | AbD13504 gallery image 2

Chicken PBMCs (stimulated for 3 days with concanavalin A) stained with Human anti Chicken CD25:FITC (HCA173F)

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CD25 Antibody | AbD13504 gallery image 3

Chicken PBMCs (stimulated for 3 days with concanavalin A) stained with Human anti Chicken CD25:Alexa Fluor®647 (HCA173A647)

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  • CD25 Antibody | AbD13504 thumbnail image 1
  • CD25 Antibody | AbD13504 thumbnail image 2
  • CD25 Antibody | AbD13504 thumbnail image 3
  • Human anti Chicken CD25:Alexa Fluor® 647
  • Human anti Chicken CD25:FITC
  • Human anti Chicken CD25
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AbD13504
  • Isotype
    HuCAL Fab bivalent
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA173FFdatasheet pdfdatasheet pdf0.1 mg
    HCA173F
    HCA173Fdatasheet pdfdatasheet pdf0.1 mg
    HCA173
    HCA173A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    HCA173A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Human anti Chicken CD25 antibody, clone 13504 recognizes the chicken IL-2 Receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature.
      Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
      CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
      Chicken IL-2Rα is the low affinity receptor for Interleukin-2.
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Duckno
      Gooseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to Alexa Fluor 647 (A647) - liquid.
      A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.
      A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinantly expressed his-tagged chicken CD25
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.05mg/ml
      Antibody concentration 0.1mg/ml
      Antibody concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
      Phosphate buffered saline.
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD25 Antibody Formats

    Formats Clone Applications Sizes available
    CD25 Antibody : Purified AbD13504 F 0.1 mg
    CD25 Antibody : FITC AbD13504 F 0.1 mg
    CD25 Antibody : Alexa Fluor® 647 AbD13504 F 100 Tests/1ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti Histidine Tag:DyLight®549MCA1396D549GA0.1 mgF, IF
      MCA1396D549GA
      Mouse anti Histidine Tag:DyLight®800MCA1396D800GA0.1 mgWB
      MCA1396D800GA
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647HCA052A6471 mlE, F
        HCA052A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative Control:FITCHCA052F0.1 mgC, E, F, P, WB
        HCA052F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD4:FITCMCA2164F0.1 mgF
          MCA2164F
          Mouse anti Chicken CD8 Alpha:FITCMCA2166F0.1 mgF
          MCA2166F
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA
          Mouse anti Chicken CD8 Alpha:RPEMCA2166PE0.1 mgF
          MCA2166PE
          Mouse anti Chicken CD4:RPEMCA2164PE100 TestsF
          MCA2164PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA
          Mouse anti Chicken CD8 Alpha:RPEMCA2166PE0.1 mgF
          MCA2166PE
          Mouse anti Chicken CD4:RPEMCA2164PE100 TestsF
          MCA2164PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA

          Recommended Positive Controls

            Histology Controls

              References

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