Cathepsin L

//www.bio-rad-antibodies.com/cathepsin-l-antibody-kit-ict941.html
Cathepsin L gallery image 1

Staining of staurosporine-induced THP-1 cells using the Magic Red Cathepsin L kit

Enlarge
  • Cathepsin L thumbnail image 1
  • Magic Red™ Cathepsin L Kit
  • Magic Red™ Cathepsin L Kit
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    ICT942IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf100 Tests
    ICT942
    ICT941IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf25 Tests
    ICT941
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • The Magic Red Cathepsin L kit uses a quick and easy method to analyze intracellular Cathepsin L protease activity in whole living cells.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Magic Red MR-(FR)2 Reagent - lyophilized
      Hoechst 33342 Stain, 1 mL
      Acridine orange (AO), 0.5 mL
      Magic Red MR-(FR)2 Reagent - lyophilized
      Hoechst 33342 Stain, 1 mL
      Acridine orange (AO), 0.5 mL
    • Preparing The Antibody
    • Test Principle
      Cathepsin Magic Red kits measure Cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.

      The Magic Red reagent contains a Cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.

      The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

      This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
      Cathepsin Magic Red kits measure Cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.

      The Magic Red reagent contains a Cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe.

      The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

      This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
    • Buffer Solution
    • Storage
      Store the unopened kit (and each unopened component) at +4oC until the expiration date.
      Protect the MR-(FR)2 reagent from light at all times.

      Once reconstituted, the 260X MR-(FR)2 stock should be stored at -20°C protected from light.
      Store the unopened kit (and each unopened component) at +4oC until the expiration date.
      Protect the MR-(FR)2 reagent from light at all times.

      Once reconstituted, the 260X MR-(FR)2 stock should be stored at -20°C protected from light.
    • Shelf Life
      Please see label for expiry date.
      Please see label for expiry date.
    • Acknowledgements
      Magic Red is a trademark of Immunochemistry Technologies, LLC.
      Magic Red is a trademark of Immunochemistry Technologies, LLC.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT942.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT941.pdf

    Additional Cathepsin L Formats

    Formats Applications Sizes available
    Cathepsin L : Magic Red™ (Red Fluorescence) IF 100 Tests | 25 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Magic Red™ Cathepsin L KitICT942100 TestsIF
          ICT942

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...

              You May Also Be Interested In...