Peptidoglycan Antibody | 3F6B3 (10H6)

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Peptidoglycan Antibody | 3F6B3 (10H6) gallery image 1

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Mouse anti peptidoglycan antibody, clone 3F6B3 (7263-1006) used for the detection of Bacillus cereus or Staphylococcus epidermidis derived biofilms by innunofluorescence.
Image caption:
verlay and fluorescent images of B. cereus (A, B), S. epidermidis (C, D) biofilms, B. canadensis (E, F) and T. rex (G, H) vessels, exposed to antibodies raised against peptidoglycan, a bacterially produced glycosaminoglycan that is a component of both bacterial cell walls and the EPS they secrete. Binding of these antibodies is evident in both biofilm products, but no binding is seen of these antibodies to either dinosaur vessel. Scale bar for all images = 20 μm

From: Citation: Schweitzer MH, Moyer AE, Zheng W (2016)
Testing the Hypothesis of Biofilm as a Source for Soft Tissue and Cell-Like Structures Preserved in Dinosaur Bone.
PLoS ONE 11(2): e0150238. doi:10.1371/journal.pone.0150238

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Mouse anti Peptidoglycan antibody used to detect spirochetes in the brain of a patient with Lyme neuroborreliosis by immunofluorescence.
Extra- and intracellular atypical and cystic forms of spirochetes in the cerebral cortex of a patient with pathologically and serologically confirmed chronic Lyme neuroborreliosis where Borrelia burgdorferi sensu stricto was cultivated from the brain. A: Colony-like agglomeration of spirochetes as revealed by monoclonal anti-OspA antibody in the cerebral cortex. B: A close up of the central part of the mass seen in A. In addition to a few helically shaped spirochetes (arrow) numerous ring-shaped forms and spherules (asterisk) are visible, which are identical to those observed in vitro following 1 week
Borrelia
exposure of primary neurons (compare with Fig. 4G). C: Spirochetes showing loop or ring-shaped formations (arrows) in the cerebral cortex immunostained with a polyclonal anti-Borrelia burgdorferi antibody (Biodesign, B65302R). They are similar to those of Treponema pallidum (arrows in D) observed in the cerebral cortex of a patient with general paresis. Immunostaining was performed using a polyclonal anti-Treponema pallidum antibody (Biodesign, B65210R). E: Helically shaped OspA immunoreactive spirochetes in the cytoplasm of a cortical pyramidal neuron. In addition to one more typical form (arrow), fine OspA positive minute granules along filamentous forms are seen. F: Intracellular ring-shaped forms (arrow) showing positive immunoreaction with a polyclonal anti-Borrelia burgdorferi antibody (BB1017). They are identical to those observed in chicken primary neurons infected with Borrelia (compare F with Figure 4D). Near the asterisk a large strongly immunoreactive cyst form is visible. Spirochete forming loop in the cerebral cortex (G) and in the cytoplasm of an epithelial cell of the choroid plexus (H) are seen as visualized by anti-OspA and anti-bacterial peptidoglycan antibodies, respectively. I: A similar atypical spirochete forming loops in the cerebral cortex as visualized with Thioflavin S. J: In an area with colony-like spirochete aggregation in addition to some typical, regularly coiled Borrelia spirochetes (arrow) OspA positive cystic forms (asterisk) are seen. K: In the cerebral cortex near the colony-like spirochetal agglomerate a spirochete cyst (asterisk) similar to that observed in vitro is visible (compare it with Figure 5 G-J). Immunostaining was performed using a monoclonal anti-OspA antibody. Bars: A = 20 μm; B-J = 10 μm, K = 5 μm.

From: Miklossy J, Kasas S, Zurn AD, McCall S, Yu S, McGeer PL.
Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis.
J Neuroinflammation. 2008 Sep 25;5:40.

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Mouse antipeptidoglycan antibody, clone 3F6B3 used for the identification of peptidoglycan containing cells in normal and Chron's disease tissue by immunofluorescence.
Image caption:
APCs in the NAP pathway show defective PD-L1 expression in Crohn's disease.
(A) Representative fluorescent micrographs showing M cells (identified by glycoprotein 2 staining; yellow) in the epithelium overlaying lymphoid patches in Crohn's disease and control sections. Scale bars are 50?μm. (B) Typical confocal micrographs demonstrating the presence of peptidoglycan (Pg; magenta) in nanomineral positive cells (identified by calcein; green) in both Crohn's disease and control sections. Scale bars are 10?μm. (C) Example confocal micrographs of Crohnμs disease and control ileal lymphoid patch cells showing that APCs from control tissue are both nanomineral (calcein-stained, green) and PD-L1 (red) positive, but Crohn's disease associated APCs are nanomineral positive and PD-L1 negative. Scale bars are 10?μm. (D) Percentage of nanomineral (calcein-stained) and PD-L1 positive cells in control and Crohn's disease tissue (Crohn’s disease versus control: p?<?0.0004 using the Mann-Whitney test). Data are shown as mean?+?SD. In all cases samples were tested from 6 independent tissues for Crohn's disease and 9 independent tissues for control tissues. In total 177 calcein (nanomineral) positive cells were identified in the Crohn's disease samples, and 191 calcein (nanomineral) positive cells in the control tissues.

From: Robertson J, Haas CT, Pele LC, Monie TP, Charalambos C, Parkes M, Hewitt RE,Powell JJ.
Intestinal APCs of the endogenous nanomineral pathway fail to express PD-L1 in Crohn's disease.
Sci Rep. 2016 May 26;6:26747.

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  • Mouse anti Peptidoglycan
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3F6B3 (10H6)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7263-1006C, E, P *datasheet pdfdatasheet pdf0.1 ml
    7263-1006
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Peptidoglycan antibody, clone 3F6B3 recognizes the 3D polymer complex structure of peptidoglycan (PG). In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors; muramyldipeptide, N-acetylglucosamine, chitin and acid hydrolyzed chitin. The epitope appears to consist of discontinuous glycan and/or amino acid residues.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Ascitic Fluid - raw
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present.
    • Immunogen
      This antibody was raised against insoluble peptidoglycan obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      Treatment with strong acid, for Gram positive bacteria, or with a detergent, such as SDS, for Gram-negative bacteria may be necessary to expose the epitope.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Peptidoglycan Antibody Formats

    Formats Clone Applications Sizes available
    Peptidoglycan Antibody : Ascites 3F6B3 (10H6) C, E, P * 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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