Baby Rabbit Complement

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Published customer image:
Baby rabbit serum used as a source of complement in a complement bactericidal assay.
Image caption:
Killing of S. Typhimurium by total IgG, IgM and IgA from HIV-uninfected and HIV-infected sera.
Log growth phase S. typhimurium D23580 was added to a mixture of the purified antibodies and 75% BRS. The log10 proportion of the starting number of bacteria remaining after 180 minutes was determined on LB agar. To obtain total antibody of each isotype (IgG, IgM and IgA), serum was incubated sequentially with combinations of human IgA and IgM affinity matrices and protein G affinity matrix to remove IgA, IgM and IgG respectively. Sera used were HIV-uninfected (HIV-ve bactericidal, n = 3), HIV-infected bactericidal (HIV+ve bactericidal, n = 4) and HIV-infected inhibitory (HIV+ve inhibitory, n = 5) sera. Each line represents killing by purified total IgG, IgM or IgA antibodies from one serum. Data shown are from a single representative experiment, where each of the three independent experiments was performed in technical triplicates.

From: Goh YS, Necchi F, O'Shaughnessy CM, Micoli F, Gavini M, Young SP, et al. (2016)
Bactericidal Immunity to Salmonella in Africans and Mechanisms Causing Its Failure in HIV Infection.
PLoS Negl Trop Dis 10(4): e0004604.

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  • Baby Rabbit Complement
  • Baby Rabbit Complement
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  • Product Type
    Serum & Cells
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    C12CA.1FN*, IYdatasheet pdfdatasheet pdf1 ml
    C12CA.1
    C12CAFN*, IYdatasheet pdfdatasheet pdf2 ml
    C12CA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Baby rabbit complement serum preparation is intended for use as a source of rabbit complement for cytotoxicity studies.
    • Intended Use
    • Product Form
      Baby rabbit serum - lyophilised
      Baby rabbit serum - lyophilised
    • Reconstitution
      Pack Size: 1 mlReconstitute with 1 ml ice cold distilled water
      Pack Size: 2 mlReconstitute with 2ml ice cold distilled water
      Pack Size: 1 mlReconstitute with 1 ml ice cold distilled water
      Pack Size: 2 mlReconstitute with 2ml ice cold distilled water
    • Preparation
    • Preservative Stabilisers
      None present.
      None present.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC for 1 hour or aliquot and store at -70oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the product. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC for 1 hour or aliquot and store at -70oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the product. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from the date of despatch.
      6 months from the date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
      Functional Assays(1)
      (1)
      This product is not sold as sterile but can be sterilized by filtration if necessary. It is preferable to dilute the complement to a final working concentration before filtration in order to minimize loss of volume.
    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
      Functional Assays(1)
      (1)
      This product is not sold as sterile but can be sterilized by filtration if necessary. It is preferable to dilute the complement to a final working concentration before filtration in order to minimize loss of volume.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
      Use within one hour of reconstitution, keeping on ice throughout.

    Additional Baby Rabbit Complement Formats

    Formats Applications Sizes available
    Baby Rabbit Complement : Serum FN*, IY 2 ml | 1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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