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Mumps Virus

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Native Mumps Virus

Product Type
Antigen
Specificity
Mumps Virus

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PIP014
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Native mumps virus preparation Native mumps virus contains a high concentration of virus and viral components. The preparation also contains some cellular material suspended in tissue culture medium (1969B).

Mumps virus is a member of the Paramyxoviridae family and is the causative agent of mumps. Mumps is most typically characterised by painful swelling of the salivary glands and fever. In some adult men, the virus might also infect the testicles, rarely causing sterility. The virus is spread by airborne droplets from the nose or throat and can have an incubation period of up to three weeks. Infected individuals are contagious from approximately 6 days before until about 9 days after the onset of symptoms. The disease is self-limiting and treatment involves controlling specific symptoms. An effective vaccine exists and may be given separately or as part of the MMR vaccine.

Target Species
Viral
Product Form
Inactivated Mumps virus - liquid
Preparation
Mumps virus, Enders strain, cultured in BSC-1 cells. Optimally infected cells are harvested, disrupted by sonication in culture medium and subjected to low speed centrifugation. The supernatant from the infected culture is concentrated by crossflow ultrafiltration. The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.
Buffer Solution
Culture medium 1969B
Preservative Stabilisers
None present
Activity
Antigenic activity is 51.1% of internal reference batch
Approx. Protein Concentrations
Current, batch-specific concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
PIP014 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.

References for Mumps Virus

  1. Bhoj, V.G. et al. (2016) Persistence of long-lived plasma cells and humoral immunity in individuals responding to CD19-directed CAR T-cell therapy.
    Blood. 128 (3): 360-70.
  2. Riggenbach, M.M. et al. (2021) Mumps virus-specific immune response outcomes and sex-based differences in a cohort of healthy adolescents.
    Clin Immunol. 234: 108912.
  3. Böröcz, K. et al. (2022) Dynamic Features of Herd Immunity: Similarities in Age-Specific Anti-Measles Seroprevalence Data between Two Countries of Different Epidemiological History.
    J Clin Med. 11(4):1145.

UniProt
P21277
GO Terms
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005198 structural molecule activity
GO:0019013 viral nucleocapsid
GO:0030430 host cell cytoplasm
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