Influenza A H1N1 Antigen
This particular strain of influenza is a reassortant virus that includes genetic components of the H1N1 influenza pandemic swine flu in a culture-adapted virus that has been selected for its qualities as a vaccine-producing strain. The live reassortant virus has passed safety testing in ferrets, in which it is attenuated for pathogenicity relative to wild-type A/California/4/2009 virus. Because A/California/4/2009 is identified as a variant strain of a pandemic virus, live derivatives must be handled under BSL2+ facilities and procedures.
Native influenza H1N1 antigen preparation (PIP021) contains a high concentration of viral antigens as well as some egg proteins from allantoic fluid.
Hemaglutination endpoint assay; 40960 HA Units/ml
- Target Species
- Product Form
- Inactivated Influenza A H1N1 antigen - liquid
- Influenza strain A/New Caledonia/20/99 (H1N1) cultured in embryonated chicken eggs. Eggs are infected by injection into the allantoic chamber, incubated for 72 hours, and refrigerated overnight prior to harvest. Allantoic fluids are harvested from the eggs and pooled. The antigen preparation is inactivated by gamma radiation.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- 40960 HA units/ml as determined by hemagglutination endpoint assay.
This product has been rendered inactive by gamma irradiation. However this material should still be handled as infectious and should be disposed of appropriately.
- Approx. Protein Concentrations
- Total protein concentration 1.86 mg/ml
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
- 18 months from date of despatch
- For research purposes only
Applications of Influenza A H1N1 Antigen
|Application Name||Verified||Min Dilution||Max Dilution|
- Instructions For Use
- A precipitate may form upon thawing, due to high product concentration. Dilute prior to clarification or other manipulation.
Product Specific References
References for Influenza A H1N1 Antigen
Sakamoto, H. et al. (2015) A Novel Optical Biosensing System Using Mach-Zehnder-Type Optical Waveguide for Influenza Virus Detection.
Appl Biochem Biotechnol. Oct 24. [Epub ahead of print]