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Clostridium difficile Toxoid B

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Clostridium difficile Toxoid B

Product Type
Antigen
Specificity
Clostridium difficile Toxoid B

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MPP024
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Clostridium difficile is a gram-positive bacteria naturally present in the gut of some children and very few adults. Occasionally, the use of antibiotics to treat other infections may lead to C. difficile infection and severe diarrhoea and fever. Symptoms are caused by toxin A (an enterotoxin) and toxin B (a cytotoxin).

MPP024 is a highly purified, non toxic preparation derived from Clostridium difficile Toxin B. Antigenicity of this preparation is maintained to be no less than 90% of original toxin strength as measured by direct ELISA.

For a preparation of Toxoid A, see MPP022.

Target Species
Bacterial
Product Form
Inactivated toxoid derived from purified Clostridium difficile Toxin B - lyophilized
Reconstitution
Reconstitute with 250 µl distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2 µg/ml compared to active toxin lethal dose of 4 pg/ml.
Buffer Solution
0.05M HEPES, 0.15M NaCl, 5% Sucrose
Preservative Stabilisers
None present
Purity
>95% by SDS-PAGE
Approx. Protein Concentrations
0.2 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
1 month from date of reconstitution if stored at +4oC

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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