Clostridium difficile Toxoid B
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Clostridium difficile is a gram-positive bacteria naturally present in the gut of some children and very few adults. Occasionally, the use of antibiotics to treat other infections may lead to C. difficile infection and severe diarrhoea and fever. Symptoms are caused by toxin A (an enterotoxin) and toxin B (a cytotoxin).
MPP024 is a highly purified, non toxic preparation derived from Clostridium difficile Toxin B. Antigenicity of this preparation is maintained to be no less than 90% of original toxin strength as measured by direct ELISA.
For a preparation of Toxoid A, see MPP022.
MPP024 is a highly purified, non toxic preparation derived from Clostridium difficile Toxin B. Antigenicity of this preparation is maintained to be no less than 90% of original toxin strength as measured by direct ELISA.
For a preparation of Toxoid A, see MPP022.
Product Details
- Target Species
- Bacterial
- Product Form
- Inactivated toxoid derived from purified Clostridium difficile Toxin B - lyophilized
- Reconstitution
- Reconstitute with 250 µl distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2 µg/ml compared to active toxin lethal dose of 4 pg/ml.
- Buffer Solution
- 0.05M HEPES, 0.15M NaCl, 5% Sucrose
- Preservative Stabilisers
- None present
- Purity
- >95% by SDS-PAGE
- Approx. Protein Concentrations
- 0.2 mg/ml after reconstitution
Storage Information
- Storage
- This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended. - Guarantee
- 1 month from date of reconstitution if stored at +4oC
More Information
- Regulatory
- For research purposes only
Applications of Clostridium difficile Toxoid B
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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