Clostridium difficile Toxoid A
MPP022 is a highly purified, non toxic preparation derived from Clostridium difficile Toxin A. Antigenicity of this preparation is maintained to be no less than 90% of original toxin strength as measured by direct ELISA.
For a preparation of Toxoid B, see MPP024.
- Target Species
- Product Form
- Inactivated toxoid derived from purified Clostridium difficile Toxin A - lyophilized
- Reconstitute with 250 µl distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Inactivated using formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2µg/ml compared to active toxin lethal dose of 0.2ng/ml.
- Buffer Solution
- 0.05M HEPES, 0.15M NaCl, 5% Sucrose
- Preservative Stabilisers
- None present
- >95% by SDS-PAGE
- Approx. Protein Concentrations
- 0.2 mg/ml after reconstitution
- This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
- 1 month from date of reconstitution if stored at +4oC
- For research purposes only
Applications of Clostridium difficile Toxoid A
|Application Name||Verified||Min Dilution||Max Dilution|
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- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra