Bio-Rad has used HuCAL® technology to develop heat-stable antibodies that are suitable to develop robust malaria diagnostic tools for using in the tropical climates and remote areas.*
Figure 1: Thermal denaturation curve for anti-LDH (P.falciparum) antibody HCA158 measured by UV adsorption at 250 nm. The melting temperature is 81.5°C
We offer three antibodies that were generated against Plasmodium LDH (Lactate Dehydrogenase) from P. vivax and P. falciparum. And two antibodies were raised against HRP II (Histidine Rich Protein II) from P. falciparum.
These HuCAL®-based fully human monoclonal antibodies have these advantages:
HuCAL® technology was used to generate unique thermostable antibodies with the goal of suitability for superior diagnostic assay development. The recombinant phage display library was subjected to stress conditions (heat and denaturants) prior to antibody selection.
This specialized guided selection process resulted in antibodies which exhibited thermostability T(m) up to 80°C as measured with CD spectroscopy.
Table 2: Antibody Pairs for ELISA
Five HuCAL antibodies were selected based on their high performance as matched pairs for sandwich ELISA to detect LDH from P. vivax and P. falciparum and HRPII antigen from P. falciparum.
Figure 2: Sandwich ELISA for P. falciparum HRPII using antibodies HCA160 (capture) and HCA159 (detection)
Figure 2 shows a sandwich ELISA with electro-chemiluminescence detection using the MSD Sector Imager instrument.
Human anti P. falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from P. falciparum) was titrated at the indicated concentrations. Detection was performed with ECL-labelled Human anti P. falciparum HRPII (HCA159) antibody