Antibodies for Malaria Diagnostics

Bio-Rad has used HuCAL® technology to develop heat-stable antibodies that are suitable to develop robust malaria diagnostic tools for using in the tropical climates and remote areas.*

thermal denaturation curve for anti-LDH (P.falciparum) antibody

Figure 1: Thermal denaturation curve for anti-LDH (P.falciparum) antibody HCA158 measured by UV adsorption at 250 nm. The melting temperature is 81.5°C

We offer three antibodies that were generated against Plasmodium LDH (Lactate Dehydrogenase) from P. vivax and P. falciparum. And two antibodies were raised against HRP II (Histidine Rich Protein II) from P. falciparum.

These HuCAL®-based fully human monoclonal antibodies have these advantages:

  • Heat stable, thermostability T(m) up to 80°C
  • Highly specific, matched antibody pairs suitable for sandwich ELISA
  • Reproducible performance
  • Secure and consistent long-term supply
  • Antibodies available to detect both LDH and HRP II for developing reliable malaria diagnostic tools

Selection of Antibodies with High Thermostability

HuCAL® technology was used to generate unique thermostable antibodies with the goal of suitability for superior diagnostic assay development. The recombinant phage display library was subjected to stress conditions (heat and denaturants) prior to antibody selection.

This specialized guided selection process resulted in antibodies which exhibited thermostability T(m) up to 80°C as measured with CD spectroscopy.

Highly Specific Antibodies to detect LDH and HRP II

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    HuCAL antibodies selected based on their high performance as matched pairs for sandwich ELISA

    Table 2: Antibody Pairs for ELISA

    Matched Antibody Pairs for Sandwich ELISA

    Five HuCAL antibodies were selected based on their high performance as matched pairs for sandwich ELISA to detect LDH from P. vivax and P. falciparum and HRPII antigen from P. falciparum.

    sandwich ELISA with electro-chemiluminescence detection

    Figure 2: Sandwich ELISA for P. falciparum HRPII using antibodies HCA160 (capture) and HCA159 (detection)

     

     

     

     

     

    Figure 2 shows a sandwich ELISA with electro-chemiluminescence detection using the MSD Sector Imager instrument.

    Human anti P. falciparum HRPII (HCA160) was used as the coating antibody. The antigen (recombinant HRPII from P. falciparum) was titrated at the indicated concentrations. Detection was performed with ECL-labelled Human anti P. falciparum HRPII (HCA159) antibody