CD115 antibody | 604B5 2E11

Rat anti Mouse CD115

Product Type
Monoclonal Antibody
Clone
604B5 2E11
Isotype
IgG1
Specificity
CD115

Product Code Applications Pack Size List Price Your Price Qty
MCA1898GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD115 antibody, clone 604B5 2E11 recognizes the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. Murine CD115 is a 958 amino acid 110kDa single pass type I transmembrane glycoprotein with tyrosine kinase activity and acts as a membrane receptor for M-CSF.

CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Rat anti Mouse CD115 antibody, clone 604B5 2E11 has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Raw 264 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Source Reference

  1. Shadduck, R.K. et al. (1993) Preparation of a monoclonal antibody directed against the receptor for murine colony-stimulating factor-1.
    Exp Hematol. 21 (4): 515-20.

References for CD115 antibody

  1. Shadduck, R.K. et al. (1996) Paradoxical stimulation of normal and leukemic rat hematopoiesis by monoclonal antibody to CSF-1 receptor.
    Exp Hematol. 24 (2): 314-7.
  2. Gilmore, G.L. & Shadduck, R.K. (1995) Inhibition of day-12 spleen colony-forming units by a monoclonal antibody to the murine macrophage/monocyte colony-stimulating factor receptor.
    Blood. 85 (10): 2731-4.
  3. MacDonald, K.P. et al. (2005) The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion.
    J Immunol. 175 (3): 1399-405.
  4. Buono, M. et al. (2010) Sulfatase modifying factor 1-mediated fibroblast growth factor signaling primes hematopoietic multilineage development.
    J Exp Med. 207 (8): 1647-60.
  5. Weingärtner, O. et al. (2011) Differential effects on inhibition of cholesterol absorption by plant stanol and plant sterol esters in apoE-/- mice.
    Cardiovasc Res. 90: 484-92.
  6. Hayashi, Y. et al. (2010) Lumican is required for neutrophil extravasation following corneal injury and wound healing.
    J Cell Sci.123: 2987-95.
  7. Herold, S. et al. (2008) Lung epithelial apoptosis in influenza virus pneumonia: the role of macrophage-expressed TNF-related apoptosis-inducing ligand.
    J Exp Med. 205: 3065-77.
  8. Pillai, M.M. et al. (2009) Inducible transgenes under the control of the hCD68 promoter identifies mouse macrophages with a distribution that differs from the F4/80 - and CSF-1R-expressing populations.
    Exp Hematol. 37: 1387-92.
  9. View The Latest Product References
  10. Sasmono, R.T. et al. (2007) Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1.
    J Leukoc Biol. 82: 111-23.
  11. Dioszeghy V et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
    J Immunol. 181 (9): 6514-24.
  12. Fancke B et al. (2008) M-CSF: a novel plasmacytoid and conventional dendritic cell poietin.
    Blood. 111 (1): 150-9.
  13. van Dommelen, S.L. et al. (2006) Perforin and granzymes have distinct roles in defensive immunity and immunopathology.
    Immunity. 25 (5): 835-48.
  14. Scheller, M. et al. (1999) Altered development and cytokine responses of myeloid progenitors in the absence of transcription factor, interferon consensus sequence binding protein.
    Blood. 94 (11): 3764-71.
  15. O'Dea, K.P. et al. (2009) Mobilization and margination of bone marrow Gr-1high monocytes during subclinical endotoxemia predisposes the lungs toward acute injury.
    J Immunol. 182 (2): 1155-66.
  16. Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
    J Exp Med. 204 (12): 3037-47.
  17. Juss, J.K. et al. (2012) Functional redundancy of class I phosphoinositide 3-kinase (PI3K) isoforms in signaling growth factor-mediated human neutrophil survival.
    PLoS One. 7 (9): e45933.

Flow Cytometry

Synonyms
c-fms
RRID
AB_2085220
UniProt
P09581
Entrez Gene
Csf1r
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004714 transmembrane receptor protein tyrosine kinase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0019955 cytokine binding
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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