Blog Topic: Tips & Tricks

26 blog(s) on Tips & Tricks available

Stain-Free vs. No-Stain — Western Blotting Mythbusting

Stain-Free vs. No-Stain — Western Blotting Mythbusting

Discover the real differences between Stain-Free and No-Stain western blotting. Compare speed, sensitivity, workflow, and equipment to choose the best method.

Sample Preparation in Flow Cytometry — Tips & Tricks

Sample Preparation in Flow Cytometry — Tips & Tricks

In this blog, we provide tips and advice on generating a high-quality sample for flow cytometry.

How to Build Complex Multicolor Flow Cytometry Panels

How to Build Complex Multicolor Flow Cytometry Panels

In this blog, we’ve compiled our top tips and steps to follow when designing multicolor flow cytometry panels.

Operation Titration: Tips for Titrating Your Antibodies

Operation Titration: Tips for Titrating Your Antibodies

In this blog, we discuss best practices for titrating your antibodies for flow cytometry.

Top Tips for Presenting Your Flow Data

Top Tips for Presenting Your Flow Data

In this blog, we cover some key considerations to keep in mind when presenting your data, with a particular focus on the differences between an external and internal audience.

Tips for Writing Your Thesis

Tips for Writing Your Thesis

In this blog we’ve compiled our top tips to make your writing process as easy (or at least tolerable) as possible.

Getting Started in Science Writing and Communication

Getting Started in Science Writing and Communication

In this blog, Lab Crunches Editor, Kimberley Bryon-Dodd shares her journey from scientist to science communicator and her top tips for getting started in this field.

Treat Them Right! Best Practices for Storing and Working with Antibodies

Treat Them Right! Best Practices for Storing and Working with Antibodies

This blog discusses some best practices for storing and using your antibodies to get the most out of them in your experiments.

The Training Bench: Workout Your Immunoprecipitation Fitness Goals

The Training Bench: Workout Your Immunoprecipitation Fitness Goals

If your IP technique is something that you are trying to improve, this blog has advice to help you to get better immunoprecipitation results.

6 Steps for Success with Multicolor Flow Cytometry

6 Steps for Success with Multicolor Flow Cytometry

In this blog, we cover some key considerations for multicolor flow cytometry panel design, and highlight handy tools to help you get great results from the offset.

How to Use Flow Cytometry to Measure Apoptosis: Part Two

How to Use Flow Cytometry to Measure Apoptosis: Part Two

Design robust experiments and troubleshoot existing protocols for measuring apoptosis by flow cytometry, by following the advice in this blog.

3 Reasons to Use alamarBlue In Your Cell Proliferation Studies

3 Reasons to Use alamarBlue In Your Cell Proliferation Studies

In this blog, we will explain what alamarBlue is and how it works, and then explore three main benefits of this reagent.

Lessons from the Pandemic: How to Be a Good Lab Mate

Lessons from the Pandemic: How to Be a Good Lab Mate

In this guest blog, Sabine shares the research challenges caused by the pandemic and how her lab has implemented small changes to how they work for the benefit of the whole research group.

How to Pull-Down Your Target Protein and Interactors Successfully

How to Pull-Down Your Target Protein and Interactors Successfully

This blog will take you through the basics of IP so you can get successful results from the outset.

How to Avoid Cell Stress While Cell Sorting

How to Avoid Cell Stress While Cell Sorting

This blog describes the forces that cells undergo during sorting, and practical steps you can take to minimize cell stress and get great results in your downstream applications, like RNA sequencing.

9 Tips to Get Publication Ready Flow Plots

9 Tips to Get Publication Ready Flow Plots

In this blog, our Flow Cytometry Research Associate shares 9 top tips to generate publication-worthy flow plots in a format that reviewers would like to see.

4 Ways to Organize Your Way to Better Science

4 Ways to Organize Your Way to Better Science

This blog contains our top tips to help you be more organized and do the best possible science.

Top Tips for Troubleshooting Your BrdU Blues

Top Tips for Troubleshooting Your BrdU Blues

This blog post focuses on BrdU and top tips and tricks for conducting a BrdU labeling experiment to help you troubleshoot any BrdU blues.

A Guide to IHC-paraffin Success

A Guide to IHC-paraffin Success

This blog is written to help you perfect your IHC-paraffin technique.  It focuses on some of the key stages so that you can achieve publication-quality results.

SUMOylation: a Heavyweight in the Regulation of Cell Cycle Progression and Cell Proliferation – Part One

SUMOylation: a Heavyweight in the Regulation of Cell Cycle Progression and Cell Proliferation – Part One

This blog post discusses the discovery of SUMO and the importance of SUMOylation in cell cycle regulation and cellular proliferation.

SUMOylation: a Heavyweight in the Regulation of Cell Cycle Progression and Cell Proliferation – Part Two

SUMOylation: a Heavyweight in the Regulation of Cell Cycle Progression and Cell Proliferation – Part Two

We examine SUMOylation, the effect of their aberrant SUMOylation on signaling pathways and the potential of SUMO inhibitors in cancer therapy.

Bromodeoxyuridine (BrdU) in adult neurogenesis research - Part 2

Bromodeoxyuridine (BrdU) in adult neurogenesis research - Part 2

Our second part of BrdU in adult neurogenesis research, describes “the dark side of BrdU” and how you can carefully control BrdU labeling experiments

Bromodeoxyuridine (BrdU) in adult neurogenesis research - Part 1

Bromodeoxyuridine (BrdU) in adult neurogenesis research - Part 1

The first of a two part series describing how BrdU revolutionized the field of neurogenesis.

Applying best practices for characterizing critical reagents

Applying best practices for characterizing critical reagents

This blog post defines critical reagents and best practices for their characterization.

“Is it the antibody?” Tips for caring for your antibody for consistent results

“Is it the antibody?” Tips for caring for your antibody for consistent results

With over 35 years of experience with antibodies, we offer our expert advice for taking care of your antibodies for consistent results.

Measuring the inevitable divide - tools for assessing cell proliferation

Measuring the inevitable divide - tools for assessing cell proliferation

This post describes crucial markers and useful markers to study aberrant cell proliferation.