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SpyLock Technology
Explained
Spylock technology is a new approach to bispecific screening based on SpyTag technology. When paired with the Pioneer Antibody Discovery Platform, SpyLock technology emerges as an ideal solution for swiftly generating bispecific antibodies tailored for high-throughput screening.
The Bio-Rad team has developed a groundbreaking innovation called SpyLock - a 'lockable' SpyCatcher that can be reversibly inhibited to prevent unwanted ligation with SpyTag. Reactivity is restored by reducing the disulfide bond and removing the small molecule inhibitor.
The Bio-Rad team has developed a groundbreaking innovation called SpyLock - a 'lockable' SpyCatcher that can be reversibly inhibited to prevent unwanted ligation with SpyTag. Reactivity is restored by reducing the disulfide bond and removing the small molecule inhibitor.
How Spylock Works
A SpyCatcher-SpyLock dimer, the BiLockCatcher, reacts with the first SpyTagged antibody fragment (A in the figure). Then by “unlocking” the second SpyLock site using a reducing agent, the second SpyTagged antibody fragment (B) can be loaded to produce a bispecific antibody.
Benefits of SpyLock Technology
Speed
- No need to construct an expression vector if coupled with Pioneer Antibody Discovery Platform
- Bispecific generation and purification is as fast as 90 minutes
Purity
- Controlled SpyTag/SpyCatcher reaction enables simple purification
- Bispecific products can be >99% pure
Versatility
- Only a single antibody format needed: SpyTagged antibody fragments
- Easily scalable, and high throughput compatible
"The rapid generation of a large number of bsAb prototypes in the BiLockCatcher format streamlines the preliminary functional screening process. SpyLock technology minimizes the need for converting bsAbs into the final format, significantly reducing workload and accelerating candidate selection.”
SpyLock Technology is detailed in the publication by Bio-Rad R&D team on Nature Communications (2024) 15:5939). doi.org/10.1038/s41467-024-50296-y.
Engineered reversible inhibition of SpyCatcher reactivity enables rapid generation of bispecific antibodies
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