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SpyDisplay Technology
Explained
SpyDisplay is a proprietary selection technology, harnessing SpyTag methodology. It enables a faster and more efficient selection process for discovering high-affinity antibodies.
SpyTag technology leverages an autocatalytic reaction between SpyTag, a 13-amino-acid peptide, and SpyCatcher, a 12.3 kDa protein, forming a stable isopeptide bond. SpyTag and SpyCatcher are derived from the fibronectin-binding protein of Streptococcus pyogenes. This reaction is rapid, typically occurring within minutes to an hour, and remains specific and efficient across a range of pH values (5–8), buffer conditions, and even in the presence of detergents.
How SpyDisplay Works
SpyDisplay uses a phagemid-based expression vector which encodes a SpyTagged Fab and a SpyCatcher-pIII integrated into the E. coli genome. In SpyDisplay, the Fab is covalently displayed via SpyTag/SpyCatcher ligation on the filamentous phage carrying the SpyCatcher fused to the pIII coat protein.
Advantages of SpyDisplay
- Same vector for both selection and expression stages, less errors as no subcloning step required
- High probability of correctly folded and displayed Fab due to absence of Fab-pIII fusion
- Resulting antibodies carry a SpyTag, compatible with TrailBlazer Antibody Platform
- Monovalent display system capable of selecting antibodies with sub-nM affinities
- Polyvalent display possible for antibodies against difficult targets
SpyDisplay Technology is detailed in the publication by Bio-Rad R&D team on mAbs 15 (1). doi:10.1080/19420862.2023.2177978.
SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology
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