Ocrelizumab antibody | AbD50641ad
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ocrelizumab Antibody, clone AbD50641ad (TZA044) at a concentration of 2 μg/ml followed by an HRP-labelled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with ocrelizumab, rituximab, infliximab, human IgG1 kappa control (same allotype as ocrelizumab and rituximab) or human lambda control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ocrelizumab Antibody, clone AbD50641ad (TZA044) titrated to the given concentrations in PBST followed by an HRP-labelled anti-DYKDDDDK tag antibody in HISPEC diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Figure A. PE conjugated ocrelizumab pre-incubated for 1h with Human Anti-Ocrelizumab Antibody, clone AbD50641ad (TZA044, red) or recombinant Human IgG1 Kappa Allotype G1m3 Antibody, clone AbD18705_hIgG1 isotype control (HCA192G, grey) in the monovalent Fab format in ratio 1:1.
Figure B. PE conjugated ocrelizumab pre-incubated for 1h with Human Anti-Ocrelizumab Antibody, clone AbD50641ad (TZA044, red) or Human IgG1 Kappa Allotype G1m3 Antibody, clone AbD18705_hIgG1 isotype control (HCA192G, grey) in the monovalent Fab format in ratio 5:1.
All experiments were performed on a Daudi cell line expressing CD20 and incubated for 1 hour with the antibody mix.
A microtiter plate was coated overnight with Human Anti-Ocrelizumab Antibody, clone AbD50641ad (TZA044) at a concentration of 0.1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of ocrelizumab. Detection was performed using HRP conjugated Human Anti-Ocrelizumab Antibody, clone AbD50651pap (TZA045P) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Ocrelizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD50641ad
- Isotype
- Fab antibody
- Specificity
- Ocrelizumab
Antibody Quality and Performance Guarantee
TZA044
| Human Anti-Ocrelizumab Antibody, clone AbD50641ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug ocrelizumab and its biosimilar products. Consequently, this antibody can be used to measure free ocrelizumab levels in serum from patients. This antibody is available in two formats - a monovalent Fab unconjugated format, clone AbD50641ad (TZA044), which has a SpyTag incorporated. This format can be used as a capture antibody in a pharmacokinetic (PK) bridging ELISA together with AbD50651 as a detection antibody. Clone AbD50641 is also available in full length IgG1 format under product code HCA400 which can be used in an ADA bridging ELISA as a surrogate positive control. Human Anti-Ocrelizumab Antibody, clone AbD50641ad has been shown to cross-react with the antibody drug rituximab (tradename Rituxan). Ocrelizumab is the humanized form of rituximab. There are changes in at least one of the CDRs and it contains Fc modification. Ocrelizumab (trade name Ocrevus) is a monoclonal IgG1/kappa antibody (VH3/κ1). In vitro, it binds to CD20 cell surface molecules on target cells thereby inducing immune effector mechanisms such as antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and apoptosis. In vivo, ocrelizumab selectively and effectively depletes CD20+ B cells. Hence, it is an immunosuppressive drug. Ocrelizumab is used to treat primary progressive multiple sclerosis and relapsing forms of multiple sclerosis in adults (including clinically isolated syndrome, relapsing-remitting disease, and active secondary progressive disease). |
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- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from Bio-Rad’s human antibody phage display libraries. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Ocrelizumab
- Affinity
- The monovalent intrinsic affinity of Human anti-Ocrelizumab Antibody, clone AbD50641ad was measured as KD<0.001 nM by real time, label-free molecular interaction analysis on immobilized ocrelizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Ocrevus is a registered trademark of Genentech/ Roche and Biogen.
Rituxan is a registered trademark of Genentech and Biogen. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA.
Clone AbD50641ad can be used as the capture antibody for ocrelizumab in a PK bridging ELISA together with clone AbD50651pap (TZA045P) as the detection antibody.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyTag3 Peptide | BLP086 | IY | 1 mg |
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| Description | SpyTag3 Peptide | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Ocrelizumab | HCA399 | E | 0.1 mg |
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab | HCA400 | E | 0.1 mg |
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab | HCA401 | E | 0.1 mg |
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab | TZA045 | E | 0.1 mg | Log in | |||
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab:HRP | TZA045P | E | 0.1 mg | Log in | |||
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| Description | Human anti Ocrelizumab:HRP | ||||||
| BiSpyCatcher2:HRP | TZC002P | IY | 1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | BiSpyCatcher2:HRP | ||||||
- Synonyms
- Ocrevus
TZA044
166857
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