HA-Tag antibody
Rabbit anti HA-Tag
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- HA-Tag
Rabbit anti HA-Tag antibody recognizes the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors. Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Haemagglutinin epitope were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Keyhole limpet hemocyanin conjugated epitope tag peptide YPYDVPDYA (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Paraffin 1 | |||
Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
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TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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Further Reading
-
Field, J. et al. (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
Mol Cell Biol. 8 (5): 2159-65. -
Wilson, I.A. et al. (1984) The structure of an antigenic determinant in a protein.
Cell. 37 (3): 767-78.
- Synonyms
- Hemagglutinin Tag
- RRID
- AB_808596
- UniProt
- Q03909
- GO Terms
- GO:0016021 integral to membrane
- GO:0019031 viral envelope
- GO:0019064 viral envelope fusion with host membrane
- GO:0020002 host cell plasma membrane
- GO:0046789 host cell surface receptor binding
- GO:0055036 virion membrane
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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