Cytosine Deaminase Antibody

//www.bio-rad-antibodies.com/yeast-cytosine-deaminase-antibody-2485-4906.html?RenderPageSpecific=1
Cytosine Deaminase Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • Cytosine Deaminase Antibody thumbnail image 1
  • Sheep anti Yeast Cytosine Deaminase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2485-4906E, WBdatasheet pdfdatasheet pdf1 ml
    2485-4906
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Human cytosine deaminase antibody recognizes cytosine deaminase, also known as cytosine aminohydrolase. Dytosine deaminase is a 158 amino acid  kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognises the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004).
    • Intended Use
    • Target Species
      Yeast
    • Product Form
      Purified Ig - liquid
    • Reconstitution
    • Preparation
      Purified Ig prepared by affinity chromatography on Protein G
    • Antiserum Preparation
      Antisera to cytosine deaminase were raised by repeated immunisations sheep of with highly purified antigen.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Purified yeast cytosine deaminase.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      zinc ion binding
      nucleus
      cytosine deaminase activity
      cytidine metabolic process
      cytosine metabolic process
      pyrimidine-containing compound salvage
      cytoplasm
      identical protein binding
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/2500
      Western Blotting1/1001/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      In Western blot two bands were visualised by ECL at 13kD and 17kD.
    • Instructions For Use

    Additional Cytosine Deaminase Antibody Formats

    Formats Applications Sizes available
    Cytosine Deaminase Antibody : Purified E, WB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

              Write your review

              You may also be interested in...