VIVAFIX™ Assay

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VIVAFIX™ Assay gallery image 1

Excellent separation between live and dead cells using the VivaFix Cell Viability Assay

Jurkat cells were stained with the VivaFix 498/521 Cell Viability Assay fixed with 3.7% formaldehyde (A) or not fixed (B), and analyzed with the S3™ Cell Sorter. SSC, side scatter

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  • VIVAFIX™ Assay thumbnail image 1
  • VIVAFIX™ 410/450 Cell Viability Assay
  • VIVAFIX™ 583/603 Cell Viability Assay
  • VIVAFIX™ 498/521 Cell Viability Assay
  • VIVAFIX™ 398/550 Cell Viability Assay
  • VIVAFIX™ 353/442 Cell Viability Assay
  • VIVAFIX™ 408/512 Cell Viability Assay
  • VIVAFIX™ 547/573 Cell Viability Assay
  • VIVAFIX™ 649/660 Cell Viability Assay
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
8 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1351112Fdatasheet pdfdatasheet pdf200 Tests
    1351112
    1351117Fdatasheet pdfdatasheet pdf200 Tests
    1351117
    1351115Fdatasheet pdfdatasheet pdf200 Tests
    1351115
    1351114Fdatasheet pdfdatasheet pdf200 Tests
    1351114
    1351111Fdatasheet pdfdatasheet pdf200 Tests
    1351111
    1351113Fdatasheet pdfdatasheet pdf200 Tests
    1351113
    1351116Fdatasheet pdfdatasheet pdf200 Tests
    1351116
    1351118Fdatasheet pdfdatasheet pdf200 Tests
    1351118
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • VivaFix cell viability assays are easy-to-use, versatile solutions for assessing the viability of mammalian cells by flow cytometry and microscopy. In most flow cytometry experiments, the exclusion of dead cells within a sample is an important step to prevent erroneous interpretation of the data caused by nonspecific fluoresence signals. When performing cell imaging experiments, cell viability and live:dead ratio are parameters that are frequently evaluated to measure cell health.

      The proprietary dyes in the VivaFix Cell Viability Assay can easily assist researchers with distinguishing betweeen live and dead cells by covalently binding to primary amines. In live cells, the VivaFix Dyes bind to the cell surface primary amines. In dead cells, where the plasma membrane is compromised, the dyes are able to permeate the cell and also react with intracellular primary amines. As a result, a greater number of fluorophores is associated with dead cells and at least a 100-fold difference in fluorescence intensity is measured between the live and dead cells thereby allowing an easier discrimination between two populations. VivaFix Dyes are compatible with cell fixation without any significant loss of fluorescence.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
      Kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only
      Store at -20oC only
      Store at -20oC only
      Store at -20oC only
      Store at -20oC only
      Store at -20oC only
      Store at -20oC only
      Store at -20oC only
    • Shelf Life
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note 1: Any buffer without sodium azide, serum or protein can be used instead of phosphate buffered saline.

      Note 2: For multicolor experiments, the cells can be stained with the antibodies of your choice before or after staining with VivaFix Dye. Antibody staining conditions should be optimized for your assay.

      1. Prepare a 500x stock solution by adding 50 μl of dimethyl sulfoxide (DMSO) to a VivaFix Dye vial and mix by vortexing.

      2. Wash cells once with phosphate buffered saline, then resuspend cells at 2-3 x 106 cells/ml in phosphate buffered saline. Use 0.5 ml of cell suspension per assay.

      3. Add 1 μl of 500x dye stock solution (from step 1) to 0.5 ml cells/assay and mix by vortexing.

      4. Incubate the mixture for 30 min at room temperature (RT) or in a 37oC/5% CO2 incubator. Protect from light.

      Note: The optimal dye concentration and incubation time for different cell lines should be assessed empirically.

      5. Wash cells twice in phosphate buffered saline.

      6. Fix cells as desired. For 3.7% formaldehyde:

      -Suspend cells in 900 μl of phosphate buffered saline following step 5

      -Add 100 μl of 37% of formaldehyde to the cell suspension

      -Incubate for 15 min at room temperature, then wash cells twice with phosphate buffered saline

      7. Resuspend cells in the appropriate flow analysis buffer.

      8. Sort cells with an S3e Cell Sorter™, analyze cells with a flow cytometer, view and capture an image of cells with the ZOE™ Fluorescent Cell Imager, or view cells with a conventional fluorescence microscope.

    Additional VIVAFIX™ Assay Formats

    Formats Applications Sizes available
    VIVAFIX™ Assay : 410/450 F 200 Tests
    VIVAFIX™ Assay : 398/550 F 200 Tests
    VIVAFIX™ Assay : 649/660 F 200 Tests
    VIVAFIX™ Assay : 583/603 F 200 Tests
    VIVAFIX™ Assay : 353/442 F 200 Tests
    VIVAFIX™ Assay : 408/512 F 200 Tests
    VIVAFIX™ Assay : 547/573 F 200 Tests
    VIVAFIX™ Assay : 498/521 F 200 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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