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Yellow Fever Virus

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  • Recombinant Yellow Fever Virus NS1 Protein
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP052AEdatasheet pdfdatasheet pdf100 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant yellow fever virus NS1 protein is produced as a hexamer in the human 293 cell line. This product has been purified and retains its native folding state and post-translational modifications providing optimal antigenicity. Research indicates that the hexamer is the biologically active form of the NS1 antigen and therefore involved in the pathogenesis of yellow fever virus (YFV).

      Yellow fever virus belongs to the Flaviviridae family and like closely related viruses, such as West Nile virus, is an abovirus utilizing various mosquito species as vectors. YFV appears to be restricted naturally to human and other primates but can be experimentally induced in other mammalian species. In recent years an increasing number cases of YFV infection have been reported.

      Originating in Africa, YFV has been introduced to tropical regions of South America and periodic outbreaks have historically occurred in both Europe and North America. According to the World Health Organization (WHO), it has been estimated that approximately 200,000 people contract YFV annually with around 30,000 cases resulting in fatality, the majority of these on the African continent.

      This product may be used in research to investigate vaccine development (Bonaldo, M.C. et al. 2014) or in research into the development of assays to detect YFV infection (Ding, X.X,et al. 2014).
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - liquid
    • Reconstitution
    • Preparation
      Recombinant yellow fever virus NS1 protein, sequence strain 17D, expressed in 293 human cells
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
      Approximate protein concentration 0.48 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Dulbecco's phosphate buffered saline
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Yellow Fever Virus Formats

    Formats Applications Sizes available
    Yellow Fever Virus : Rec. Protein E 100 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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