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Mouse anti Yellow Fever virus antibody, clone 2D12 used for the detection of yellow fever envelope protein in infected dendritic cells by flow cytometry.
Monocyte derived dendritic cells (MoDCs) infection with dengue virus 2 (DENV2) and yellow fever (YF) 17DD. MoDCs were infected with DENV2 or YF17DD at equivalent multiplicities of infections (MOIs) [2.5 x 105 50% tissue culture infectious dose (TCID)/ml] or YF17DD at higher MOI (1.25 x 106 TCID50/ml) or mock infected. Cells were labelled with anti DENV or anti YF antibodies using triplicates for each peripheral blood mononuclear cell (PBMC) donor. A: flow cytometry patterns for viral antigens on gated MoDCs 24 h after viral infection. Histograms demonstrate cell distribution by Alexa Fluor-488 fluorescence intensity (FL1-H: level of fluorescence). Cells were cultured with mock (dark grey) or DENV2 or YF17DD (light grey) at concentrated (conc) or equivalent (equiv) doses; B: viral antigen detection kinetics on infected MoDcs (data from four PBMC donors) [hpi: hours post-infection; X axis: time after infection (hours); Y axis: percentage of viral antigen positive cells (Ag+)]; C: intracellular viral antigen detection 24h after infection representing 11 cell donors (horizontal lines: distribution mean; X axis: viral inoculum; Y axis: percentage of viral Ag+ cells. Asterisks mean: p<0.0001 in one way analysis of variance and Tukey&s multiple comparison test).
From: Gandini M, Reis SR, Torrentes-Carvalho A, Azeredo EL, Freire Mda S, Galler R, Kubelka CF. Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-a and IFN-a profiles. Mem Inst Oswaldo Cruz. 2011 Aug;106(5):594-605.