Yellow Fever Virus Antibody | 2D12 (0G5)

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Yellow Fever Virus Antibody | 2D12 (0G5) gallery image 1

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Mouse anti Yellow Fever virus antibody, clone 2D12 used for the detection of yellow fever envelope protein in infected dendritic cells by flow cytometry.
Image caption:
Monocyte derived dendritic cells (MoDCs) infection with dengue virus 2 (DENV2) and yellow fever (YF) 17DD. MoDCs were infected with DENV2 or YF17DD at equivalent multiplicities of infections (MOIs) [2.5 x 105 50% tissue culture infectious dose (TCID)/ml] or YF17DD at higher MOI (1.25 x 106 TCID50/ml) or mock infected. Cells were labelled with anti DENV or anti YF antibodies using triplicates for each peripheral blood mononuclear cell (PBMC) donor. A: flow cytometry patterns for viral antigens on gated MoDCs 24 h after viral infection. Histograms demonstrate cell distribution by Alexa Fluor-488 fluorescence intensity (FL1-H: level of fluorescence). Cells were cultured with mock (dark grey) or DENV2 or YF17DD (light grey) at concentrated (conc) or equivalent (equiv) doses; B: viral antigen detection kinetics on infected MoDcs (data from four PBMC donors) [hpi: hours post-infection; X axis: time after infection (hours); Y axis: percentage of viral antigen positive cells (Ag+)]; C: intracellular viral antigen detection 24h after infection representing 11 cell donors (horizontal lines: distribution mean; X axis: viral inoculum; Y axis: percentage of viral Ag+ cells. Asterisks mean: p<0.0001 in one way analysis of variance and Tukey&s multiple comparison test).

From: Gandini M, Reis SR, Torrentes-Carvalho A, Azeredo EL, Freire Mda S, Galler R, Kubelka CF. Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-a and IFN-a profiles. Mem Inst Oswaldo Cruz. 2011 Aug;106(5):594-605.

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  • Mouse anti Yellow Fever Virus
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2D12 (0G5)
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9801-8006FN, IFdatasheet pdfdatasheet pdf0.1 ml
    9801-8006
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Yellow fever virus antibody, clone 2D12 recognizes the envelope protein of the wild (Asibi) and vaccine strains of yellow fever virus. The clone has been reported to have neutralising activity against the Asibi strain (Schlessinger et al. 1984). No cross reactivity with other flaviviruses has been reported.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Ascites - Liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None Present
    • Immunogen
      17D strain of yellow fever virus
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Yellow Fever Virus Antibody Formats

    Formats Clone Applications Sizes available
    Yellow Fever Virus Antibody : Ascites 2D12 (0G5) FN, IF 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Immunoprecipitation

              References

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