West Nile Virus

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  • Recombinant West Nile Virus NS1 Antigen
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP051AEdatasheet pdfdatasheet pdf100 µg
    PIP051A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant West Nile Virus NS1 Antigen is a purified preparation of the West Nile virus non-structural protein 1 (NS1).

      West Nile Virus (WNV), also known as West Nile Fever, is primarily a mosquito borne virus belonging the Flaviviridae family. In humans the majority of infections are subclinical with patients experiencing either no or very mild symptoms, however a minority of infections result in severe disease, which may result in fatal encephalitis.

      Originating in Africa, WNV was, until recently, not a signifinant disease of humans. Since the the late 1990's the virus has spread widely to other continents and is now an endemic pathogen in the temperate and tropic reagions throughout the world. WNV infection is now considered a serious health concern in North America (Lindsey et al. 2014).

      WNV is zoonotic disease with an avian wildlife resevoir to which passerine and more specifcally, corvid species appear to be particularly susceptible with high recorded mortality rates (Abdelrazec et al. 2014). In addition to the human health concerns and the effect on North American avian species populations (Wheeler et al. 2011), WNV is also of potential concern in domestic mammals, in particular to equine species where mortality rates are high (Agenvoort et al. 2013). Fatal cases of West Nile have also been noted other species including non-human primates, bats and cetaceans. While the primary route of infection is via mosquitos there is some evidence that infection may also occur from other invertebrate species such as ticks, however further research is required in this area.

      Recombinant West Nile virus NS1 antigen is presented in its native folded state complete with post-translational modifications, delivering optimal antigenicity and making it suitable for use in vaccine research and serology-based assays.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified recombinant protein - liquid
    • Reconstitution
    • Preparation
      Recombinant West Nile virus NS1 protein, sequence strain NY99, expressed in 293 human cells
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE
    • Approx. Protein Concentrations
      Approximate protein concentration 0.55 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Dulbecco's phosphate buffered saline
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional West Nile Virus Formats

    Formats Applications Sizes available
    West Nile Virus : Rec. Protein E 100 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human anti WNV NS2B-NS3HCA0850.1 mgE, WB
          HCA085
          Human anti WNV NS2B-NS3HCA085A50 µgE, WB
          HCA085A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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