SARS Spike Protein Antibody

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  • Rabbit anti SARS Spike Protein (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP889Edatasheet pdfdatasheet pdf0.1 mg
    AHP889
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti SARS Spike Protein antibody recognizes theSARS (Severe acute respiratory syndrome) Spike glycoprotein. The Spike protein is a 1242 amino acid ~135 kDa single pass type I transmembrane membrane protein and functions in adhesion to host cells, where it binds to Angiotensin-converting enzyme 2 (Turner et al. 2004).

      The amino terminal region folds to a globular shape, forming the characteristic spike structure and contains the binding site for ACE2 (Du et al 2009).
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to SARS spike were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to a sequence within the amino terminus of the SARS Spike protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      host cell surface receptor binding
      host cell endoplasmic reticulum-Golgi intermediate compartment membrane
      integral to membrane
      virion attachment, binding of host cell surface receptor
      cellular membrane fusion
      pathogenesis
      structural molecule activity
      host cell plasma membrane
      viral envelope
      transmission of virus
      virion membrane
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional SARS Spike Protein Antibody Formats

    Formats Applications Sizes available
    SARS Spike Protein Antibody : Purified E 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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