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Rubella Virus

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  • Native Rubella Virus
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP045Edatasheet pdfdatasheet pdf1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Native Rubella virus preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE buffer with 20% sucrose. Some residual non-viral protein from the host tissue is present. The preparation does not contain detergent..

      Rubella virus is an enveloped, single-stranded RNA virus of the Togaviridae family. Infection with the virus leads to rubella, commonly known as German measles, a common childhood illness. The primary symptom is a rash on the face, trunk and limbs which normally fades after about three days. Fever, swollen glands, joint pains, headaches and conjunctivitis may also be present. Symptoms are usually mild and the virus is rapidly eliminated from the body. Infection of a pregnant woman is potentially serious since the child may be born with congenital rubella syndrome, characterised by cardiac, cerebral, ophthalmic and auditory defects.

      For a Rubella virus preparation of lower purity, please see PIP044.
    • Intended Use
    • Target Species
    • Product Form
      Inactivated Rubella virus - liquid
    • Reconstitution
    • Preparation
      Vero cells infected with Rubella virus release virus particles into the culture supernatant. The supernatant is harvested, clarified and concentrated. The virus suspension is purified by two cycles of sucrose gradient purification.

      The antigen preparation is inactivated by exposure to UV light. This method leads to maximum loss of infectivity with a minimum loss of antigenicity.
    • Source
      Rubella virus, HPV-77 strain, cultured in Vero cells, an established cell line from African Green Monkey kidney.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      NTE buffer (Sodium chloride, Tris and EDTA)
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      PIP045 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats.

    Additional Rubella Virus Formats

    Formats Applications Sizes available
    Rubella Virus : Inactivated Pathogen E 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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