Negative Isotype Controls
- Native Rubella Virus preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE buffer with sucrose. Some residual non-viral protein from the host tissue is present. The preparation does not contain detergent.
Rubella virus is an enveloped, single-stranded RNA virus of the Togaviridae family. Infection with the virus leads to rubella, commonly known as German measles, a common childhood illness. The primary symptom is a rash on the face, trunk and limbs which normally fades after about three days. Fever, swollen glands, joint pains, headaches and conjunctivitis may also be present. Symptoms are usually mild and the virus is rapidly eliminated from the body. Infection of a pregnant woman is potentially serious since the child may be born with congenital rubella syndrome, characterised by cardiac, cerebral, ophthalmic and auditory defects.
For a more highly purified Rubella virus preparation, please see PIP045.
Inactivated Rubella virus - liquid
Vero cells infected with Rubella virus release virus particles into the culture supernatant. The supernatant is harvested, clarified and concentrated. The virus suspension is purified by one cycle of sucrose gradient purification.
The antigen preparation is inactivated by exposure to UV light. This method leads to maximum loss of infectivity with a minimum loss of antigenicity.
Rubella virus, HPV-77 strain, cultured in Vero cells, an established cell line from African Green Monkey kidney.
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 1.10 mg/ml.
Reagents In The Kit
Preparing The Antibody
NTE buffer (Sodium chloride, Tris and EDTA) + sucrose buffer
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
18 months from date of despatch.
For research purposes only
Additional Rubella Virus Formats
Recommended Secondary Antibody
Recommended Negative Isotype Control
Recommended Positive Controls