Respiratory Syncytial Virus Antibody | 4 clone blend

//www.bio-rad-antibodies.com/viral-respiratory-syncytial-virus-antibody-4-clone-blend-7950-1705.html?RenderPageSpecific=1
Respiratory Syncytial Virus Antibody | 4 clone blend gallery image 1

Published customer image:
Mouse anti Respiratory syncytial virus antibody, 4 clone blend (7950-1705) used for the identification of RSV inthe lungs of infected hispid cotton rats (Sigmodon hispidus) by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Histopathological findings of lung tissues following infections with the RSV Long strain. (A) The results of hematoxylin-eosin (HE) staining and immunostaining are shown. Lung tissues were obtained from cotton rats inoculated with the supernatant of the HEp-2 cell homogenate through an intranasal route as a negative control (a). Lung tissues were obtained four days after the first, second, third, and fourth infections and the results of HE staining are shown (b-e). Lung tissues were stained with a four-clone blend of monoclonal antibodies against the RSV P, F, and N proteins and anti-mouse IgG conjugated with HRP (f-i). Each panel shows the magnification of 1:200. (B) Lung tissues infected with the Long strain were scored. Lung tissues were sectioned and stained with HE. Six random fields per group were scored for histopathology based on the following criteria: narrowing alveolar space, thickness of alveolar walls, destruction of bronchial epithelial cells, peribronchial infiltration of inflammatory cells, mucus production, and bleeding. Bars represent means ± SE.

From: Yamaji Y, Yasui Y, Nakayama T (2016)
Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats.
PLoS ONE 11(5): e0155777.

Enlarge
Respiratory Syncytial Virus Antibody | 4 clone blend gallery image 2

Published customer image:
Mouse anti Respiratory syncytial virus antibody, 4 clone blend (7950-1705) used for the identification of RSV inthe lungs of infected hispid cotton rats (Sigmodon hispidus) by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Histopathological findings after third and fourth infections with wild-type RSV subgroups A and B. (A) Cotton rats were infected with RSV/A/Tokyo/2012 or RSV/B/Tokyo/2012 in the second or third infections with the Long strain. The results of HE staining after infections with RSV/A/Tokyo/2012 (a and b), and those of immunostaining after infections with RSV/A/Tokyo/2012 were shown (c and d). The results of HE staining after infections with RSV/B/Tokyo/2012 were shown (e and f), and those of immunostaining after infections with RSV/B/Tokyo/2012 were shown (g and h). Each panel shows a magnification of 1:200. (B) Lung tissues infected with RSV/A/Tokyo/2012 or RSV/B/Tokyo/2012 were scored. Bars represent means ± SE.

From: Yamaji Y, Yasui Y, Nakayama T (2016)
Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats.
PLoS ONE 11(5): e0155777.

Enlarge
  • Respiratory Syncytial Virus Antibody | 4 clone blend thumbnail image 1
  • Respiratory Syncytial Virus Antibody | 4 clone blend thumbnail image 2
  • Mouse anti Respiratory Syncytial Virus
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4 clone blend
  • Isotype
    IgG1IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7950-1705C, IF*, P *datasheet pdfdatasheet pdf0.25 ml
    7950-1705
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Respiratory Syncitial Virus antibody recognizes Respiratory syncytial virus (RSV), an enveloped virus belonging to the Paramyxoviridae, genus Pneumovirus, that causes minor respiratory infections in adults and bronchitis and bronchopneumonia in children. Its genome consists of single stranded, negative sense RNA that codes for 2 non-structural and 8 structural proteins.

      Mouse anti Respiratory Syncitial Virus antibody recognizes the RSV phosphoprotein, fusion and nuclear protein and reacts with human respiratory syncytial virus subtypes A and B.

      Mouse anti Respiratory Syncitial Virus antibody does not react with tissue culture isolates of influenza types A and B, parainfluenza types 1, 2, 3 & 4b, adenovirus, HSV types1 & 2, VZV, cytomegalovirus, mumps and measles virus, coxsackie B4 virus, poliovirus types 1, 2 & 3 or Echo virus 19.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      RSV strain A2.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/501/400
      Immunofluorescence(1)1/51/20
      Immunohistology - Paraffin(2)1/501/400
      (1)
      Acetone fixation is recommended.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      RSV infected lung.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Respiratory Syncytial Virus Antibody Formats

    Formats Clone Applications Sizes available
    Respiratory Syncytial Virus Antibody : S/N 4 clone blend C, IF*, P * 0.25 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              RSV infected lung.

              References

              Write your review

              You may also be interested in...