Parainfluenza Virus 2

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  • Native Parainfluenza Virus 2
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  • Product Type
    Antigen
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP016Edatasheet pdfdatasheet pdf1 ml
    PIP016
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Native Parainfluenza Virus 2 contains a high concentration of virus and viral components. The preparation also contains some cellular material suspended in Tissue Culture Media.

      Parainfluenza virus 2 is one of four serotypes of parainfluenza viruses in the paramoxyvirus family. Parainfluenza viruses are the second most common cause of lower respiratory tract infections in young children. Parainfluenza virus 2 causes upper and lower respiratory tract illnesses and croup.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Inactivated Parainfluenza virus 2 - liquid
    • Reconstitution
    • Preparation
      Optimally infected monolayers are harvested, disrupted by sonication and subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated using crossflow ultrafiltration with a 30,000 molecular cutoff.

      The antigen preparation is inactivated using gamma radiation.
    • Source
      Parainfluenza virus 2, Greer strain, cultured in Vero cells.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 2.02 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Tissue Culture Media
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      This product should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.

    Additional Parainfluenza Virus 2 Formats

    Formats Applications Sizes available
    Parainfluenza Virus 2 : Inactivated Pathogen E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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