Papillomavirus 16 Oncoprotein E7 Antibody | 716-D1

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  • Mouse anti Human Papillomavirus 16 Oncoprotein E7
  • Mouse anti Human Papillomavirus 16 Oncoprotein E7
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    716-D1
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2818E, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2818
    MCA2818GE, WBdatasheet pdfdatasheet pdf1 mg
    MCA2818G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Papillomavirus 16 Oncoprotein E7 antibody, clone 716-D1 recognizes both the monomer and dimer forms of the E7 oncoprotein of human papilloma virus 16. Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupts normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection.

      Mouse anti Human Papillomavirus 16 Oncoprotein E7 antibody, clone 716-D1 cross-reacts with both the monomer and dimer forms of the E7 protein of HPV-18.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium azide
      0.09% Sodium azide
    • Immunogen
      Oncoprotein E7 from human papillomavirus 16.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      intracellular
      protein binding
      metal ion binding
      DNA binding
      regulation of transcription, DNA-dependent
      sequence-specific DNA binding transcription factor activity
      evasion by virus of host immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Papillomavirus 16 Oncoprotein E7 Antibody Formats

    Formats Clone Applications Sizes available
    Papillomavirus 16 Oncoprotein E7 Antibody : Purified 716-D1 E, WB 1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad

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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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