Papillomavirus 16 L1 Late Protein Antibody | CamVir-1

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Papillomavirus 16 L1 Late Protein Antibody | CamVir-1 gallery image 1

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Mouse anti Papillomavirus 16 L1 Late Protein antibody, clone CamVir-1 used for the identification of the L1 late protein in viral preparations by western blotting.
Image caption:
Verification of self-assembly and subsequent in vitro biotinylation of HPV16 Avi-L1 VLPs a Purification of HPV16 Avi-L1 VLPs (HI) VLPs was performed by ultracentrifugation (UC) on an iodixanol (OptiprepTM) density-gradient (27%/33%/39%).
Subsequent reduced SDS-PAGE analyses showed the presence of a 56kDa protein band (theoretical size of Avi-L1) in the high-density UC fractions (4–6) containing particulate material. b Transmission-electron microscopy (TEM) analysis of material representing UC fraction 4 post UC purification. To verify the integrity of the chimeric HPV16 Avi-L1 (HI) VLPs, an aliquot of diluted particles was placed on carbon-coated grids, negatively stained with 2% phosphotungstic acid (pH = 7.0) and examined by transmission electron microscopy (TEM) using a CM 100 BioTWIN at magnification x 36,000 (Å), scale bar 80 nm. c Western blot analysis of fraction 4 post UC purification. The blot demonstrates the presence of HPV16 Avi-L1 (56kDa) detected by Camvir-1 (lane one) and successful biotinylation of HPV16 Avi-L1 using Strep-HRP to detect biotin (lane two).

From: Thrane S, Janitzek CM, Agerbæk MØ, Ditlev SB, Resende M, Nielsen MA, et al. (2015) A Novel Virus-Like Particle Based Vaccine Platform Displaying the Placental Malaria Antigen VAR2CSA. PLoS ONE 10(11): e0143071.

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Papillomavirus 16 L1 Late Protein Antibody | CamVir-1 gallery image 2

Published customer image:
Mouse anti Papillomavirus 16 L1 Late Protein antibody, clone CamVir-1 used for the identification of the L1 late protein in viral preparations by western blotting.
Image caption:
HPV16 Avi-L1 VLP coupled to mSA-ID1-ID2a analyzed by ultracentrifugation followed by SDS-PAGE, Western blot and TEM analysis.
a). After coupling of the mSA-ID1-ID2a antigen to the HPV16 Avi-L1 VLPs, excess antigen was removed by UC over an OptiprepTM gradient (27%/33%/39%). Reducing SDS-PAGE analysis showed the presence of two protein bands corresponding to the size of HPV16 Avi-L1 (56kDa) and mSA-VAR2CSA (85kDa), respectively, in the high-density fractions (4–6) post UC purification. Excess unbound mSA-VAR2CSA was present in the higher UC fractions (12–14) containing soluble proteins. b). Transmission electron microscopy (TEM) analysis of material from UC fraction 4 containing HPV16 Avi-L1 VLPs coupled with mSA-VAR2CSA. An aliquot of diluted particles was placed on carbon-coated grids, negatively stained with 2% phosphotungstic acid (pH = 7.0) and examined by transmission electron microscopy (TEM) using a CM 100 BioTWIN at magnification x 36,000 (Å). Black scale bar 200 nm, enhanced section white scale bar 40 nm. c Western blot analysis of fraction 4 post UC purification of mixed HPV16 Avi-L1 and mSA-VAR2CSA. The blot confirms the presence of HPV16 Avi-L1 and mSA-VAR2CSA detected by Camvir-1 and a-PENTA HIS-tag, respectively.

From: Thrane S, Janitzek CM, Agerbæk MØ, Ditlev SB, Resende M, Nielsen MA, et al. (2015) A Novel Virus-Like Particle Based Vaccine Platform Displaying the Placental Malaria Antigen VAR2CSA. PLoS ONE 10(11): e0143071.

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  • Mouse anti Papillomavirus 16 L1 Late Protein
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CamVir-1
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7135-2804IF, IP, P, WBdatasheet pdfdatasheet pdf0.1 mg
    7135-2804
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Papilloma virus 16 L1 late protein antibody, clone CamVir-1 recognizes papilloma virus and reacts with a late protein of HPV type 16. Can be used to detect HPV in human biopsies and smears. HPV-6 and -11 positive biopsies gave negative results.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Amino acids 198-531 of HPV 16-L1 fused to beta-galactosidase.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      structural molecule activity
      viral capsid
      host cell nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      Paraffin embedded, acetone or paraformaldehyde fixed sections are suitable.
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Detects a 56 kDa band corresponding to the L1 protein. It also detects a 41 kDa protein in uninfected mammalian cells. This unwanted specificity is absent in IPPT/IHC.
    • Instructions For Use

    Additional Papillomavirus 16 L1 Late Protein Antibody Formats

    Formats Clone Applications Sizes available
    Papillomavirus 16 L1 Late Protein Antibody : Purified CamVir-1 IF, IP, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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