Metapneumovirus Antibody | HMPV123

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Metapneumovirus Antibody | HMPV123 gallery image 1

A Newcastle strain of HMPV grown on LLC MK cells (A) and non infected cells (B) stained with Mouse anti Metapneumovirus, MCA4671 (green), and counterstained with Evans Blue (red)

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  • Metapneumovirus Antibody | HMPV123 thumbnail image 1
  • Mouse anti Metapneumovirus
  • Mouse anti Metapneumovirus
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HMPV123
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4671IFdatasheet pdfdatasheet pdf0.2 mg
    MCA4671
    MCA4671GIFdatasheet pdfdatasheet pdf1 mg
    MCA4671G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Metapneumovirus antibody, clone HMPV123 recognizes human metapneumovirus (hMPV), a member of the Paramyxoviridae RNA virus family. Metapneumovirus was discovered in 2001 and is distributed worldwide. Infection in the upper respiratory tract is associated with mild illness, while lower respiratory tract infection can cause fever, cough, pneumonia, bronchiolitis and wheezing. Severe lower respiratory tract infection is most common in the very young, the very old and the immunosuppressed. Metapneumovirus is a common cause of respiratory infection.

      Phylogenetic analysis has shown two major genotypes, A and B, each containing two sub-divisions, suggesting that four distinct lineages circulate in the population with different lineages predominating in different years.

      Mouse anti Metapneumovirus antibody, clone HMPV123 recognizes the nucleoprotein of all sub-types (A1, A2, B1 and B2) of hMPV and may be used in conjunction with clones HMPV57, HMPV33 and HMPV24 to detect all strains of hMPV.

      Mouse anti Metapneumovirus antibody, clone HMPV123 does not cross-react with cell cultures infected with hRSV, influenzaviruses A and B, adenovirus, parainfluenza viruses 1, 2, 3 and 4b, mumps virus, measles virus, varicella-zoster virus, herpes simplex virus types 1 and 2, human cytomegalovirus, human herpesvirus type 6, ECHOvirus 19, Coxsachievirus B4, poliovirus types 1-3, HHV6 or uninfected HeLa, MA104, 3MK, LLC-MK, HEp2, MRC-5 and HSB-2 cells. It also does not react with sputum bacteria.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Pack Size: 1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Sub-type A hMPV virus isolate NCL03-4/145.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/401/80
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/401/80

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Metapneumovirus Antibody Formats

    Formats Clone Applications Sizes available
    Metapneumovirus Antibody : Purified HMPV123 IF 1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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