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Isav Antibody | 4A11

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  • Mouse anti Isav
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5977C, E, IFdatasheet pdfdatasheet pdf0.5 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti ISAV, clone 4A11, specifically recognizes the Infectious Salmon Anemia Virus, an orthomyxovirus being the sole member of the Isavirus genus. ISAV is the causative agent for Infectious Salmon Anemia (ISA), a notifiable disease affecting Atlantic salmon and resulting in high mortality (Weli, S.C., et al. 2013). ISAV is similar in structure to other orthomyxoviruses, with the exception of the hemagglutinin (HA) protein that would appear to be significantly different in size than for other influenza type viruses (Rimstad, E., et al. 2001)

      ISAV was first identified in farmed salmon in Norway in 1984, spread by wild marine fish species, it has since been detected in farmed Atlantic salmon stocks throughout the North Atlantic, North West America and more recently in Chile, where it has also been detected in farmed Coho salmon (Godoy, M.G., et al. 2008). Due to the high mortality of infected individuals, ISAV has a significant ecconomic impact and infections are of serious concern to the aquaculture industry.

      While the route of entry into the host, is as yet, poorly understood, evidence indicates that the virus enters through epithelial tissue in the gills (Weli, S.C., et al. 2013), this is similar to mammalian orthomyxoviruses which target respiratory tissues.

      Clone 4A11can be used to identify ISAV in the tissues of infected fish and may be of use for research into methods for diagnosis and control of ISAV infections in the aquaculture industry.
    • Intended Use
    • Target Species
    • Product Form
      Tissue Culture Supernatant - liquid (concentrated)
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      ISAV isolate
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 - 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Mouse SP2/0-Ag-14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Isav Antibody Formats

    Formats Clone Applications Sizes available
    Isav Antibody : Con S/N 4A11 C, E, IF 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Salmonid IgMCA21820.5 mlE, F, IF, WB
          Mouse anti Rainbow Trout IgMCA59760.5 mlC, E, IF, WB
          Rabbit anti Salmonid IgAHP76150 µlE, IF, WB

          Recommended Positive Controls

            Histology Controls

              Further Reading

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