Influenza B Nucleoprotein Antibody | B017 (B35G)

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Influenza B Nucleoprotein Antibody | B017 (B35G) gallery image 1

Published customer image:
Mouse anti Influenza B Nucleoprotein antibody, clone B017 used for the evaluation of viral nucleoprtien by western blotting.
Image caption:
NS1 WT but not dsRNA-binding deficient NS1 protein co-sediments with PKR and vRNP upon density gradient centrifugation.
(A) A549 cells were infected with WT virus or mutant virus #4 at an MOI of 1. Cells were lyzed 12 hours p.i. and subjected to centrifugation through a continuous 5 to 50% sucrose gradient. 16 fractions were taken from top to bottom. Fractions 1 to 9 were analyzed by immunoblotting with antibodies specific for PKR and the viral NP and NS1 proteins. Also, RNA was extracted from gradient fractions 1 to 9 and was subjected to dot blot hybridization with probes specific for HA vRNA and NS vRNA, respectively (panels “HA and NS vRNA”). Whole cell lysates were analyzed by immunoblotting with antibodies specific for phospho-PKR, total PKR, viral NP, viral NS1 and tubulin as indicated (right panel,“lysate”). (B) A549 cells were mock treated or infected with WT virus or virus mutant #4 as described in panel A. Lysates were prepared and subjected to immunoprecipitation with anti-PKR (α) or control antibody (ctrl). The precipitated complexes were analyzed by immunoblotting for PKR and NS1 proteins. RNA was isolated from an identical set of PKR immunoprecipitates of cells infected with the mutant virus and subjected to dot blot analysis with an RNA-probe specific for HA vRNA.

From: Dauber B, Martínez-Sobrido L, Schneider J, Hai R, Waibler Z, Kalinke U, et al. (2009) Influenza B Virus Ribonucleoprotein Is a Potent Activator of the Antiviral Kinase PKR. PLoS Pathog 5(6): e1000473.

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Influenza B Nucleoprotein Antibody | B017 (B35G) gallery image 2

Published customer image:
Mouse anti Influenza B Nucleoprotein antibody, clone B017 used for the evaluation of viral nucleoprtien by immunofluorescence and western blotting.
Image caption:
The nuclear egress of vRNPs late in infection leads to PKR activation.
(A) A549 cells grown on glass cover slips were infected with WT or mutant virus #4 at an MOI of 1. Cells were mock treated or complemented with LMB starting at 3 hours p.i.. At 8, 12 and 16 hours p.i., cells were fixed and stained for NP (shown in red color), and also for the NS1 protein at the 16 h time-point (shown in green color). Microscopic sample analysis was conducted by confocal laser scanning microscopy. Scale bar, 10 µm. (B) A549 cells grown in culture dishes were infected with WT virus or mutant virus #4 at an MOI of 1. Cells were mock treated or complemented with LMB starting 3 hours p.i. and lyzed 8, 12 and 16 hours p.i.. Whole cell lysates were analyzed by immunoblotting with antibodies specific for phospho-PKR, total PKR, NP, NS1 and tubulin as indicated.

From: Dauber B, Martínez-Sobrido L, Schneider J, Hai R, Waibler Z, Kalinke U, et al. (2009) Influenza B Virus Ribonucleoprotein Is a Potent Activator of the Antiviral Kinase PKR. PLoS Pathog 5(6): e1000473.

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  • Mouse anti Influenza B Nucleoprotein
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    B017 (B35G)
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA403E, IF, WBdatasheet pdfdatasheet pdf1 mg
    MCA403
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Influenza B Nucleoprotein antibody, clone B017 recognises an epitope within the nucleoprotein of influenza B virus.

      The influenza viruses, classified as type A, B and C, are members of the Orthomyxoviridae family which differ in their epidemiology and host ranges and lack serological cross-reactivity of their internal components, especially their matrix proteins and nucleoprotein. Influenza B virus is a slow-mutating single stranded RNA virus subject to antigenic drift which, although enough to prevent lasting immunity, prevents influenza B from causing pandemics.

      Mouse anti Influenza B Nucleoprotein antibody, clone B017 can be used in influenza B IFA typing in conjunction with MCA2717 (clone B114).
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Influenza B/Lee/40 and B/Singapore/-222/79 viruses.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the P3 Ag8.653 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Influenza B Nucleoprotein Antibody Formats

    Formats Clone Applications Sizes available
    Influenza B Nucleoprotein Antibody : Purified B017 (B35G) E, IF, WB 1 mg
    • Copyright © 2016 Bio-Rad

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              References

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