Influenza A H5 Antigen Antibody | 11A9

//www.bio-rad-antibodies.com/viral-influenza-a-h5-antigen-antibody-11a9-mca2660.html?RenderPageSpecific=1
Influenza A H5 Antigen Antibody | 11A9 gallery image 1

Published customer image:
Mouse anti influenza A H5 antigen antibody, clone 11A9 (MCA2660) used for the detection of H5 antigen in infected Vero cells by immunofluorescence.
Image caption:
Construction and cellular expression of influenza virus-like particles (VLPs) in mammalian cells. (A) Diagrams of mammalian expression vectors of influenza VLPs. Indicated are the CMV constitutive promoter (CMV), tetracycline repressor gene (TetR), polyadenylation signals (PA), CMV doxycycline (Dox)-inducible promoter (CMV-TO), chimeric intron (In), encephalomyocarditis virus internal ribosomal entry site (IRES), and influenza genes (M1 and M2, matrix 1 and 2; HA, hemagglutinin; NA, neuraminidase). (B), (C) Expression of viral genes in selected quadruple VLP producer cells. The total cell lysates were extracted from VLP producer cells without (-) or with (+) Dox induction, and then probed with respective specific antibodies against HA, NA, M1, and M2 in Western blot assays. Expression of N2 was detected by RT-PCR. Molecular masses of expressed HA, NA, M1, and M2 are indicated on the right. (D), (E) In vivo immunofluorescence staining of expressed viral proteins in VLP producer cells with antibodies against HA and NA (red) as marked and counterstained with DAPI (blue).

From: Wu C-Y, Yeh Y-C, Yang Y-C, Chou C, Liu M-T, Wu H-S, et al. (2010)
Mammalian Expression of Virus-Like Particles for Advanced Mimicry of Authentic Influenza Virus.
PLoS ONE 5(3): e9784.

Enlarge
Influenza A H5 Antigen Antibody | 11A9 gallery image 2

Published customer image:
Mouse anti influenza A H5 antigen antibody, clone 11A9 (MCA2660) used for the detection of H5 antigen in purified VLPs by immuno-electrom microscopy.
Image caption:
Morphology and antigen presence determinations of purified mammalian VLPs.
(A), (B) Secreted VLPs and virus were purified by sucrose density gradient ultracentrifugation, negatively stained with 2% uranyl acetate, and observed by TEM at 100,000× magnification; pictures of respective virus strains are shown below the VLPs. (C), (D) Immunogold electron microscopy of purified VLPs. The primary antibodies used are shown below individual panels. Secondary antibody was goat anti-rabbit conjugated to 12 nm gold beads.

From: Wu C-Y, Yeh Y-C, Yang Y-C, Chou C, Liu M-T, Wu H-S, et al. (2010)
Mammalian Expression of Virus-Like Particles for Advanced Mimicry of Authentic Influenza Virus.
PLoS ONE 5(3): e9784.

Enlarge
  • Influenza A H5 Antigen Antibody | 11A9 thumbnail image 1
  • Influenza A H5 Antigen Antibody | 11A9 thumbnail image 2
  • Mouse anti Influenza A H5 Antigen
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    11A9
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2660Edatasheet pdfdatasheet pdf0.2 mg
    MCA2660
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Influenza A H5 Antigen antibody, clone 11A9 detects haemagglutinin H5 from the Influenza A virus H5N1. Influenza A belongs to the Orthomyxoviridae family, and is a negative sense single-stranded RNA virus which results in respiratory disease.

      Haemagglutinin is an antigenic glycoprotein which allows viral attachment and entry into the cell. Sixteen subtypes of haemagglutinin (H1-H16) have been described, of which H1, H2 and H3 infect humans. H5 and H7 normally result in avian disease, with some highly pathogenic H5N1 strains causing 100% mortality in poultry. H5N1 has in some cases mutated to infect humans, with 60% mortality. Haemagglutinin is one of the most medically relevant antigens on influenza as it is a target for antiviral drugs and antibodies.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Purified avian influenza virus type A (H5N1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen and lymph node cells from immunised Balb/C mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Influenza A H5 Antigen Antibody Formats

    Formats Clone Applications Sizes available
    Influenza A H5 Antigen Antibody : Purified 11A9 E 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...