Influenza A H1N1 antibody
Goat anti Influenza A H1N1 polyclonal antibody (5315-0064) used to label influenza virus infected tissues in ferrets (Mustela putorius furo) by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
AAV‐HA and AAV‐cHA have a protective effect in ferrets.
IHC staining of submucosal glands (SMG) (G) or bronchi (H) with an anti‐H1N1 polyclonal antibody (bar: 100 &mum; red: influenza antigen (Neufuchsin); blue: nuclei (hematoxylin)).
From: Demminger DE, Walz L, Dietert K, Hoffmann H, Planz O, Gruber AD, von Messling V, Wolff T.
Adeno-associated virus-vectored influenza vaccine elicits neutralizing and Fcγ receptor-activating antibodies.
EMBO Mol Med. 2020 May 8;12(5):e10938.
doi: 10.15252/emmm.201910938.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
HRP conjugated Donkey anti Goat IgG antibody (OBT1500P) used to detect bound Goat anti Influenza antibody(5315-0064) by immunohistochemistry on formalin fixed, paraffin embedded influenza infected murine lung sections following heat mediated antigen retrieval in Tris/EDTA pH9.0.
Image caption:
Effect of latent gammaherpesvirus infection on moderate and severe influenza A infection. Six to eight week old BALB/c mice were infected with 5×104 pfu MHV-68 or mock-infected with BHK cell lysate and 28 days later infected with 5×102 or 5×103 pfu IAV or mock infected with DMEM. (e). Quantified total area2 of lung sections infected with IAV stained with DAB. (f). Representative images of regional IAV DAB staining in lung cross sections. All figures show data from two to three independent experiments, (N=6 or above per group). All data displayed as mean±SEM where */# P<0.05 **/## P<0.01 ***/#### P≤0.001 statistical significance unless otherwise stated.
From:Hardisty G, Nicol MQ, Shaw DJ, Bennet ID, Bryson K, Ligertwood Y, Schwarze J, Beard PM, Hopkins J, Dutia BM.
Latent gammaherpesvirus infection enhances type I IFN response and reduces virus spread in an influenza A virus co-infection model.
J Gen Virol. 2024 Feb;105(2).8 Feb [Epub ahead of print].
doi: 10.1099/jgv.0.001962.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Influenza A H1N1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Influenza A H1N1
Antibody Quality and Performance Guarantee
| Goat anti Influenza A H1N1 polyclonal antibody is specific for Influenza A virus H1N1 by Haemagglutination inhibition. This goat anti Influenza A H1N1 polyclonal antibody does not react with Influenza B, RSV, Para 1-3 or Adenovirus. It does not react with HEp-2 cells but may react with some chicken cellular proteins. Influenza type A viruses are divided into subtypes based on the antigenic differences of two viral surface proteins, hemagglutinin (H) and neuraminidase (N). On infection of the respiratory tract, the hemagglutinin molecule binds to sialic acid-containing receptors on the epithelial cells resulting in endocytosis. Once the virus has been engulfed, the hemagglutinin allows the viral membrane to fuse with the endosomal membrane. Neuraminidase functions to aid viral release from host cells by cleaving terminal sialic acid residues from carbohydrate moieties on the cell surface. Viral release also requires the interaction of the viral M1 protein with the cellular scaffold G-like protein RACK1 (Demirov et al. 2012). Subtype antigenic variations result from a process known as antigenic drift whereby these surface proteins constantly mutate in order to evade the host immune response. Subtype A(H1N1) was the cause of Spanish flu pandemic that killed approximately 50,000,000 people between 1918-1919. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Influenza A, strain USSR (H1N1)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Haemagglutination |  |
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| Immunofluorescence | |
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| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for Influenza A H1N1 antibody
-
Zielecki, F. et al. (2010) Virulence determinants of avian H5N1 influenza A virus in mammalian and avian hosts: The role of the C-terminal ESEV motif in the viral NS1 protein.
J Virol. 117: 439 - 48 -
Kash JC et al. (2011) Lethal synergism of 2009 pandemic H1N1 influenza virus and Streptococcus pneumoniae coinfection is associated with loss of murine lung repair responses.
MBio. 2(5). pii: e00172-11. -
Weinheimer, V.K. et al. (2012) Influenza A viruses target type II pneumocytes in the human lung.
J Infect Dis. 206 (11): 1685-94. -
Nicol, M.Q. et al. (2012) A novel family of peptides with potent activity against influenza A viruses.
J Gen Virol. 93: 980-6. -
Meunier, I. and von Messling, V. (2012) PB1-F2 Modulates Early Host Responses but Does not Affect the Pathogenesis of H1N1 Seasonal Influenza Virus.
J Virol. 86: 4271-8. -
Demirov, D. et al. (2012) Interaction of influenza A virus matrix protein with RACK1 is required for virus release.
Cell Microbiol. 14: 774-89. -
Kallfass, C. et al. (2013) Visualizing the beta interferon response in mice during infection with influenza A viruses expressing or lacking nonstructural protein 1.
J Virol. 87 (12): 6925-30. -
Schliehe, C. et al. (2015) The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection.
Nat Immunol. 16 (1): 67-74.
View The Latest Product References
-
Nicol, M.Q. et al. (2019) Lack of IFNγ signaling attenuates spread of influenza A virus in vivo and leads to reduced pathogenesis.
Virology. 526: 155-164. -
Demminger, D.E. et al. (2020) Adeno-associated virus-vectored influenza vaccine elicits neutralizing and Fcγ receptor-activating antibodies.
EMBO Mol Med. 12 (5): e10938. -
Goncheva, M.I. et al. (2020) Staphylococcus aureus Lipase 1 Enhances Influenza A Virus Replication.
mBio. 11 (4): e00975-20. -
Hardisty, G. et al. (2024) Latent gammaherpesvirus infection enhances type I IFN response and reduces virus spread in an influenza A virus co-infection model.
J Gen Virol. 105 (2) 8 Feb [Epub ahead of print].
- RRID
- AB_620739
5315-0064
154151
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