Influenza A Antibody | 1331

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Published customer image:
Mouse anti Influenza A nucleoprotein antibody, clone 1331 (0300-0234) used to visualize internalized virus by immunofluorescence on infected MDCK cells
Image caption:
Effect of iota-carrageenan on H1N1 virus adsorption and internalization.
(A) Adsorption. H1N1 virus was added to MDCK cells in the presence of different concentrations of iota-carrageenan or control polymer carboxymethylcellulose (CMC). After viral adsorption for 1 h at 4°C, cells were washed and the number of cell-bound infectious viral particles determined by plaque assay; red bar 400 μg/ml, orange bar 4 μg/ml iota-carrageenan, black bar 400 μg/ml, grey bar 4 μg/ml CMC. (B) Adsorption/Internalization. H1N1 virus was added to MDCK cells and adsorbed for 1 h at 4°C. Cells were washed and allowed to internalize virus in the presence or absence of different concentrations of iota-carrageenan or CMC for 1 h at 37°C. Subsequently, internalized infectious viral particles were determined by plaque assay. (C) Immunofluorescent visualisation of virus adsorption in the presence of iota-carrageenan or CMC. 1 h post adsorption at 4°C, cells were stained after 1 h at 37°C with a mouse anti-NP antibody. (D) Adsorption/Internalization. H1N1 was added to MDCK cells and adsorbed for 1 h at 4°C. Cells were washed and allowed to internalize virus in the presence of iota-carrageenan or CMC for 1 h at 37°C. Compare the bright green stainings in indicative of productive infection to 5C, where no green fluorescence is detected at high iota-carrageenan concentration.

From: Leibbrandt A, Meier C, König-Schuster M, Weinmüllner R, Kalthoff D, Pflugfelder B, et al. (2010)
Iota-Carrageenan Is a Potent Inhibitor of Influenza A Virus Infection.
PLoS ONE 5(12): e14320.

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  • Mouse anti Influenza A
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1331
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0300-0234C, E, IFdatasheet pdfdatasheet pdf0.2 mg
    0300-0234
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Influenza A antibody, clone 1331, recognizes the nucleoprotein of Influenza A. It has been found reactive with over 50 isolates from H1 through to H14 and gives 'nuclear' staining in immunofluorescence.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Influenza virus from Texas strain.
    • Purity
    • Approx. Protein Concentrations
      1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      virion
      structural molecule activity
      ribonucleoprotein complex
      host cell nucleus
      interspecies interaction between organisms
      RNA binding
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2001/2000
      Immunofluorescence1/1001/500
      Immunohistology - Frozen1/1001/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Influenza A Antibody Formats

    Formats Clone Applications Sizes available
    Influenza A Antibody : Purified 1331 C, E, IF 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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              • Application NameReference Images
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              References

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