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Goat anti Human Immunodeficiency Virus 1 gp120 antibody used for evaluation of envelope protein fragmants in HeLa cell lysates by western blotting.
ab14 enhancement of HIV-1 Env incorporation requires FIP1C and Rab14 depletion does not alter incorporation of MLV or VSV-G Env. A) Titration of Rab14Q70L in HeLa cells with normal FIP1C levels (Scr shRNA lanes) or in cells depleted of FIP1C (FIP1C shRNA lanes). Note that gp120 and gp160 blots were probed with goat anti-gp120/gp160 antisera, while gp41 was probed with murine monoclonal anti-gp41 antibody. B) Hela cells were transfected with NL4-3 or with NL4-3deltaEnv and expression constructs pHCMV-G (for VSV-G) or pCL-Ampho (MLV Env). Depletion of Rab14 was performed using shRNA in the indicated lanes. Cellular and particle Env content was assessed by immunoblotting with specific antisera for HIV gp120/160, VSV-G, or amphotropic MLV Env.
From: Qi M, Williams JA, Chu H, Chen X, Wang J-J, Ding L, et al. (2013) Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting and Particle Incorporation of the HIV-1 Envelope Glycoprotein Complex.
PLoS Pathog 9(4): e1003278.