Human Immunodeficiency Virus 1 gp120 Antibody

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Human Immunodeficiency Virus 1 gp120 Antibody gallery image 1

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Goat anti Human Immunodeficiency Virus 1 gp120 antibody used for evaluation of envelope protein fragmants in HeLa cell lysates by western blotting.
Image caption:
FIP1C depletion and HIV-1 Env incorporation in HeLa cells. (A) The efficiency of depletion of Rab11a-FIPs was measured by real time PCR (top). Infectivity of released particles following depletion of each FIP family member is shown below. (B) Depletion of FIP1C was confirmed at the protein level by immunoblotting with a FIP1C-specific antiserum. Env levels in both cell lysates and virions harvested from the FIP-depleted HeLa cells were assayed by immunoblotting. (C) Restoration of Env incorporation following shRNA-mediated depletion. Two constructs were used in this experiment: FIP1C cDNA is an shRNA sensitive GFP-tagged plasmid; while FIP1C* includes silent mutations in the shRNA target sequence rendering it shRNA-resistant. 2 ug and 4 ug of each FIP1C construct were used in the repletion assay as indicated at the top of the blot. (D) Infectivity of viral particles from the experiment shown in panel D as evaluated using TZM-bl indicator cells. Lanes are numbered and correspond to the blot above. Statistical comparisons utilized the unpaired t-test, * = p<.05; ** = p<.01.

From: Qi M, Williams JA, Chu H, Chen X, Wang J-J, Ding L, et al. (2013) Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting and Particle Incorporation of the HIV-1 Envelope Glycoprotein Complex.
PLoS Pathog 9(4): e1003278.

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Human Immunodeficiency Virus 1 gp120 Antibody gallery image 2

Published customer image:
Goat anti Human Immunodeficiency Virus 1 gp120 antibody used for evaluation of envelope protein fragmants in HeLa cell lysates by western blotting.
Image caption:
ab14 enhancement of HIV-1 Env incorporation requires FIP1C and Rab14 depletion does not alter incorporation of MLV or VSV-G Env. A) Titration of Rab14Q70L in HeLa cells with normal FIP1C levels (Scr shRNA lanes) or in cells depleted of FIP1C (FIP1C shRNA lanes). Note that gp120 and gp160 blots were probed with goat anti-gp120/gp160 antisera, while gp41 was probed with murine monoclonal anti-gp41 antibody. B) Hela cells were transfected with NL4-3 or with NL4-3deltaEnv and expression constructs pHCMV-G (for VSV-G) or pCL-Ampho (MLV Env). Depletion of Rab14 was performed using shRNA in the indicated lanes. Cellular and particle Env content was assessed by immunoblotting with specific antisera for HIV gp120/160, VSV-G, or amphotropic MLV Env.

From: Qi M, Williams JA, Chu H, Chen X, Wang J-J, Ding L, et al. (2013) Rab11-FIP1C and Rab14 Direct Plasma Membrane Sorting and Particle Incorporation of the HIV-1 Envelope Glycoprotein Complex.
PLoS Pathog 9(4): e1003278.

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  • Goat anti Human Immunodeficiency Virus 1 gp120
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2204E, WBdatasheet pdfdatasheet pdf1 mg
    AHP2204
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human Immunodeficiency Virus 1 gp120 antibody recognizes the human immunodeficiency virus envelope protein. The viral envelope protein gp160 is cleaved into fragments gp120 and gp41. gp120 is an exterior envelope glycoprotein on the surface of the virus particle that binds to CD4 and chemokine receptors on macrophages and T-lymphocytes. gp41 is non-covalently bound to gp120 and aids the virus in entering the cell.

      Goat anti Human Immunodeficiency Virus 1 gp120 antibody reacts with gp120, gp160 and gp41 in western blot assays.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/10,000
      Western Blotting1/5001/10,000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human Immunodeficiency Virus 1 gp120 Antibody Formats

    Formats Applications Sizes available
    Human Immunodeficiency Virus 1 gp120 Antibody : Purified E, WB 1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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