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Herpes simplex Virus 2

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  • Herpes simplex Virus 2
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  • Product Type
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MPP013Edatasheet pdfdatasheet pdf0.2 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Highly purified Herpes simplex Virus 2 preparation has been derived from HSV2 infected mammalian cell cultures and following viral release virions were purified by multiple density centrifugation techniques.

      Herpes simplex virus 2 (HSV2) is a member of the Herpesviridae viral family. It is a neurotropic virus that causes watery blisters on the skin or mucous membranes of humans. It is contagious and can be spread by close contact with an infected person who is producing and shedding virus from the skin. The virus is mostly latent in nerve cells of the body, but can occasionally travel to the skin, start shedding and may or may not produce the characteristic blisters. HSV1 is normally found on the genitals, but may also be associated with cold sores on the mouth or lips. There is no known cure for HSV infection and an infected person remains a carrier for life, however treatment can reduce viral shedding.

      For a cell lysate format, please see MPP012.
    • Intended Use
    • Target Species
    • Product Form
      Inactivated HSV2 - frozen
    • Reconstitution
    • Preparation
      HSV2 is propagated in mammalian cells using a protocol optimised for maximum particle yield. At the appropriate time, infected cells are washed in saline and disrupted to release virus particles by freeze thaw and sonication.

      Virus particles are purified twice by sucrose density gradients. This process also includes a DNAse step to remove cellular DNA.

      The product is inactivated by heating to 56oC for 2 hours and by the addition of Triton X-100. Inactivity is confirmed by double blind passage.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline

      0.1% Triton X-100
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Herpes simplex Virus 2 Formats

    Formats Applications Sizes available
    Herpes simplex Virus 2 : Inactivated Pathogen E 0.2 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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