Recombinant Hepatitis surface antigen preparation is a recombinant surface antigen, AY subtype of the Hepatitis B virus. It is produced using a Saccharomyces cerevisiae. expression system.
Four major serotypes of the hepatitis B virus are adentified according to variability of the surface antigen, defined by a common 'a' determinant and mutually exclusive determinant pairs 'd/y' and 'w/r'. Hence, the subtypes are adw,adr, ayw and ayr. Further subdivision of these major subtypes has identified additional minor subtypes of the virus (Magnius & Norder 1995).
Recombinant Protein - liquid
~95% by SDS PAGE
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
OBT0915 may be used in a sandwich ELISA as a standard with 4940-1404 as a capture antibody and 4940-1484 as a detection antibody.
1. Kee, G.S. et al. (2010) Exploiting the intracellular compartmentalization characteristics of the S. cerevisiae host cell for enhancing primary purification of lipid-envelope virus-like particles.Biotechnol Prog. 26 (1): 26-33.
1. Magnius, L.O. & Norder, H. (1995) Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene.Intervirology. 38 (1-2): 24-34.