Hepatitis B Surface Antigen Antibody

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Hepatitis B Surface Antigen Antibody gallery image 1

Published customer image:
Rabbit anti hepatitis B surface antigen antibody used for the evaluation of hepatitis B surface antigen in infected cell cultures by western blotting
Image caption:
(A) Materials released from Vero/hSLAM cells infected with MVvac2DIII-S,S)P or MVvac2(HBsAg)N collected 72 h after infection were clarified, and particulate material pelleted, loaded on a 20% to 60% sucrose gradient, and centrifuged to equilibrium. Five hundred microliter fractions were collected from the top (left) to the bottom (right) and weighed. The dotted line shows a representative density profile. (B) Aliquots of each fraction were separated by 12.5% SDS-polyacrylamide gel electrophoresis, immunoblotted, and probed with anti-HBsAg antibodies for fractions coming from cells infected with MVvac2DIII-S,S)P (upper panel) or MVvac2(HBsAg)N (medium panel). The lower panel indicates the migration of infectious MV virions.

From: Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
Harahap-Carrillo, I.S. et al.
Vaccines 2015; 3(3), 503-18. doi:10.3390/vaccines3030503.

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Hepatitis B Surface Antigen Antibody gallery image 2

Published customer image:
Rabbit anti hepatitis B surface antigen antibody used for the detection of hepatitis S antigen in infected mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical staining for HBsAg (A-D) and HBcAg (E-H) in liver sections. Treatment with pWPT-HBV-shRNA-1 lentivirus(C, G) or pWPT-HBV-shRNA-2 lentivirus (D, H) reduces the number of HBsAg or HBcAg-positive cells on day 7 respectively compared to the control. The staining was performed on tissue sections from three animals per group. Representative sections are shown (four sections per animals were stained and counted). Numerous HBsAg-positive and HBcAg-positive cells were seen in the empty vector group (A, E) and negative control RNAi group (B, F). No HBsAg-positive stained cells were observed in most fields in pWPT-HBV-shRNA-2 group(D). Some stained cells could be seen in pWPT-HBV-shRNA-1 or shRNA-2 groups(C, G, H) and these were indicated by arrows, but staining were much less intense than that in control groups (100× original magnification, scale bar 200 μm).

From: Deng L, Li G, Xi L, Yin A, Gao Y, You W, Wang X, Sun B. Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.
BMC Gastroenterol. 2009 Oct 6;9:73.

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Hepatitis B Surface Antigen Antibody gallery image 3

Published customer image:
Rabbit anti hepatitis B surface antigen antibody used for the detection of Hepatitis s antigen in guinea pig skin by immunohistochemistry on cryosections.
Image caption:
Plasmid expression and inflammation in the skin. Guinea pigs were treated with pHBsAg. Expression of plasmid was evaluated at 48 hrs post treatment by IHC (A-IO; B I+EP). Inflammation was measured 96 hrs post treatment and assessed by H&E (C-No treatment; D- IO, E–I+EP) at 100× magnification and 200× magnification (F- I+EP).

From: Donate A, Coppola D, Cruz Y, Heller R (2011) Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination.
PLoS ONE 6(4): e19181.

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  • Rabbit anti Hepatitis B Surface Antigen:HRP
  • Rabbit anti Hepatitis B Surface Antigen
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT0990PC, E, Pdatasheet pdfdatasheet pdf0.1 mg
    OBT0990P
    OBT0990C, E, Pdatasheet pdfdatasheet pdf1 ml
    OBT0990
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti hepatitis B surface antigen antibody recognizes Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans.

      Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Hepatitis B were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
    • Preservative Stabilisers
      0.002% Thiomersal
      1% Bovine Serum Albumin
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Hepatitis B subtypes ad and ay.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 4.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2001/1000
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,000
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a detection reagent in a sandwich ELISA together with MCA4658 as the capture reagent.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Hepatitis B Surface Antigen Antibody Formats

    Formats Applications Sizes available
    Hepatitis B Surface Antigen Antibody : Purified C, E, P 1 ml
    Hepatitis B Surface Antigen Antibody : HRP C, E, P 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen

              References

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