Hepatitis B Surface Antigen ad
Recombinant Hepatitis B surface antigen (PIP002) used in tissue culture in an in vitro cytotoxicity assay.
Image caption:
The inuence of recombinant human IL-37 to HBV peptide-induced CD8+T cell cytotoxicity in acute hepatitis B (AHB) patientsin vitro.Puried CD8+T cells from HLA-A*02 positive AHB patients (n=8) were stimulated with recombinant human IL-37 (0.1 ng/ml) for 24 h and were cocultured with HepG2.2.15 cells in both direct contact and indirect contact manners in the presence of recombinant HBsAg (10μg/ml) and HBc18-27 peptide(10μg/ml). Supernatants were harvested 48 h post coculture.
From: Liu Q, Zhou Q, Wang M, Pang B.
Interleukin-37 suppresses the cytotoxicity of hepatitis B virus peptides-induced CD8+ T cells in patients with acute hepatitis B.
Bosn J Basic Med Sci. 2022 Nov 2 [Epub ahead of print].
doi: 10.17305/bjbms.2022.8260.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Hepatitis B Surface Antigen ad
- Product Type
- Recombinant Protein
- Specificity
- Hepatitis B Surface Antigen ad
Antibody Quality and Performance Guarantee
| Recombinant Hepatitis surface antigen is a S. cerevisiae derived preparation of the AD subtype surface antigen of the Hepatitis B virus (HBsAg), a protein component of the viral envelope predominantly expressed in the cytoplasm of infected hepatocytes. HBsAg has antigenic heterogeneity; two pairs of the subtypes d/y and w/r lead to antigenic combinations of adw, ayw, adr, and ayr. Hepatitis B infection is normally diagnosed using serological tests that detect HBsAg. If HBsAg is detected in the blood for longer than six months, chronic hepatitis B is diagnosed. |
- Target Species
- Viral
- Product Form
- Recombinant Hepatitis B surface antigen AD
- Preparation
- Recombinant Hepatitis B surface antigen AD expressed in Saccharomyces cerevisiae and purified via ionic exchange chromatography, size exclusion chromatography and sterile filtration.
- Source
- S. cerevisiae
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Purity
- ~95% by SDS PAGE
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Protein Molecular Weight
- 24 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Hepatitis B Surface Antigen ad
-
Cheng, S. et al. (2017) Hepatitis B Surface Antigen Promotes the Invasion of Hepatitis B Virus-Related Hepatocellular Carcinoma Cells by Upregulation of Toll-Like Receptor 2.
Viral Immunol. 30 (3): 232-9. -
Liu, Q. et al. (2022) Interleukin-37 suppresses the cytotoxicity of hepatitis B virus peptides-induced CD8+ T cells in patients with acute hepatitis B.
Bosn J Basic Med Sci. Nov 02 [Epub ahead of print].
- Synonyms
- HBsAg
View more products with HEPATITIS B specificity
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