Hepatitis B Surface Antigen ad

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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP025Edatasheet pdf1 mg
    PIP025
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans. Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Hepatitis B surface antigen subtype ad purified from human plasma - liquid
    • Reconstitution
    • Preparation
      Hepatitis B surface antigen subtype ad was purified by three ultracentrifugation steps from human plasma. The preparation was inactivated by heat treatment for 10 hours at 60°C.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
      1) Silver stained SDS-PAGE under reducing conditions reveal two major bands corresponding to p24 and gp27. Bands corresponding to pre-S proteins, dimers and 69kDa protein also detected.

      2) 20nm spherical particles were detected by electron microscopy, as well as rod particles in some lots. Complete virions (Dane particles, 42nm) were not observed (done initially when the protocol was established).
    • Approx. Protein Concentrations
      Total protein concentration 2.64 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      -20ºC only (ship frozen).
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from the date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Hepatitis B Surface Antigen ad Formats

    Formats Applications Sizes available
    Hepatitis B Surface Antigen ad : Purified E 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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