Negative Isotype Controls
- Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans. Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
Hepatitis B surface antigen subtype ad purified from human plasma - liquid
Hepatitis B surface antigen subtype ad was purified by three ultracentrifugation steps from human plasma. The preparation was inactivated by heat treatment for 10 hours at 60°C.
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
1) Silver stained SDS-PAGE under reducing conditions reveal two major bands corresponding to p24 and gp27. Bands corresponding to pre-S proteins, dimers and 69kDa protein also detected.
2) 20nm spherical particles were detected by electron microscopy, as well as rod particles in some lots. Complete virions (Dane particles, 42nm) were not observed (done initially when the protocol was established).
Approx. Protein Concentrations
Total protein concentration 2.64 mg/ml.
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
-20ºC only (ship frozen).
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
12 months from the date of despatch.
For research purposes only
Additional Hepatitis B Surface Antigen ad Formats
Recommended Secondary Antibody
Recommended Negative Isotype Control
Recommended Positive Controls