Hepatitis A Virus

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  • Native Hepatitis A Virus
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  • Product Type
    Antigen
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PIP008Edatasheet pdfdatasheet pdf1 ml
    PIP008
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Native Hepatitis Virus preparation contains a high concentration of viral antigen, predominantly in the form of whole virions. The preparation also contains a low concentration of formaldehyde with some residual host cellular material.

      Hepatitis A virus is a non-enveloped, icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross-react with Hepatitis B virus. The virus contains four proteins VP1, VP2, VP3 and VP4.

      Hepatitis A is a wide-spread infectious disease, resulting from infection of the liver by the Hepatitis A virus (HAV). Hepatitis A infection causes jaundice, fever, anorexia, nausea, vomiting, headache and fatigue. Some cases may be fatal. The virus is mainly transmitted by the faecal-oral route via contaminated food or drinking water. The HAV is a picornavirus that replicates slowly without noticeable interference to host cell macro-molecular synthesis and, consequently, without apparent cytopathic effect. The liver pathology observed is likely to be immune-mediated.
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Inactivated Hepatitis A virus - liquid.
    • Reconstitution
    • Preparation
      Cells infected with Hepatitis A virus are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and disrupted to release viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with freon.

      The antigen preparation is inactivated by incubating with formaldehyde for 96 hours.
    • Source
      Hepatitis A virus, strain HM-175, cultured in FRhK 4 , an established cell line from foetal Rhesus monkey kidney.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.32 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      This product should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.

    Additional Hepatitis A Virus Formats

    Formats Applications Sizes available
    Hepatitis A Virus : Inactivated Pathogen E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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