Negative Isotype Controls
- Native Hepatitis Virus preparation contains a high concentration of viral antigen, predominantly in the form of whole virions. The preparation also contains a low concentration of formaldehyde with some residual host cellular material.
Hepatitis A virus is a non-enveloped, icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross-react with Hepatitis B virus. The virus contains four proteins VP1, VP2, VP3 and VP4.
Hepatitis A is a wide-spread infectious disease, resulting from infection of the liver by the Hepatitis A virus (HAV). Hepatitis A infection causes jaundice, fever, anorexia, nausea, vomiting, headache and fatigue. Some cases may be fatal. The virus is mainly transmitted by the faecal-oral route via contaminated food or drinking water. The HAV is a picornavirus that replicates slowly without noticeable interference to host cell macro-molecular synthesis and, consequently, without apparent cytopathic effect. The liver pathology observed is likely to be immune-mediated.
Inactivated Hepatitis A virus - liquid.
Cells infected with Hepatitis A virus are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and disrupted to release viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with freon.
The antigen preparation is inactivated by incubating with formaldehyde for 96 hours.
Hepatitis A virus, strain HM-175, cultured in FRhK 4 , an established cell line from foetal Rhesus monkey kidney.
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 0.30 mg/ml
Reagents In The Kit
Preparing The Antibody
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
18 months from date of despatch.
For research purposes only
Additional Hepatitis A Virus Formats
Recommended Secondary Antibody
Recommended Negative Isotype Control
Recommended Positive Controls