gp41 Antibody | AbD08066

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Human Anti-gp41 Antibody specificity ELISA

A microtiter plate was coated overnight with target antigen HIV gp41-derived chimeric construct, NCCG-gp41, and various negative control antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% milk powder, detection was performed using Human Anti-gp41 Antibody (clone AbD08066) at a concentration of 2 µg/ml and Goat Anti-Human IgG F(ab')2:Alk. Phos. Antibody (0500-0100) in HISPEC Assay Diluent (BUF049A) followed by AttoPhos AP Fluorescent Substrate.

  • gp41 Antibody | AbD08066 thumbnail image 1
  • Human anti gp41:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA239ZE, FN*datasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Human Anti-gp41 Antibody, Clone AbD08066 specifically recognizes an epitope derived from the ectodomain of the Human Immunodeficiency Virus (HIV) type 1 envelope glycoprotein 41 (gp41).

      This antibody was selected from the Human Combinatorial Antibody Library (HuCAL®) by panning on an HIV gp41-derived chimeric construct, NCCG-gp41, as described in Louis et al. 2001.

      The fusion of HIV-1 with the membrane of the host cell is the first step in the process of viral infection and replication and involves conformational changes in the gp120 and gp41 viral envelope proteins. It is now known that the HIV gp41 contains a highly conserved N-heptad repeat (N-HR) region which is conserved in many HIV-1 strains and as such it is hypothesized that this maybe a target for developing a vaccine (Gustchina et al. 2010).

      Clone AbD8066 is a unique antibody that has been shown to be broadly neutralizing across a wide panel of B and C type HIV-1 viruses. The crystal structure of the N-HR mimetic 5-Helix with this antibody has been reported (Gustchina et al. 2010, Protein Data Bank ID 3MA9).
    • Intended Use
    • Target Species
    • Product Form
      A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to a conserved internal trimeric coiled-coil of the N-heptad repeat (N-HR) of HIV-1 gp41.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays(1)
      This antibody demonstrates neutralizing properties

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional gp41 Antibody Formats

    Formats Clone Applications Sizes available
    gp41 Antibody : Preservative Free AbD08066 E, FN* 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-MH Negative ControlHCA0510.1 mgC, E, P, WB

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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