Human Anti-gp41 Antibody specificity ELISAEnlarge
A microtiter plate was coated overnight with target antigen HIV gp41-derived chimeric construct, NCCG-gp41, and various negative control antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% milk powder, detection was performed using Human Anti-gp41 Antibody (clone AbD08066) at a concentration of 2 µg/ml and Goat Anti-Human IgG F(ab')2:Alk. Phos. Antibody (0500-0100) in HISPEC Assay Diluent (BUF049A) followed by AttoPhos AP Fluorescent Substrate.